摘要
突变体是基因功能研究和品种改良的重要材料。本研究对一个中品661 EMS诱变的株型突变体(it1)进行了表型和生理鉴定,旨在为该突变体的利用提供参考。结果表明:与野生型相比,突变体株型紧凑,节间缩短,叶片变小呈深绿色且皱缩;突变体高度降低为野生型的2/3,但节间数目与野生型无显著差别,说明it1株高降低是由每个节间长度缩短造成的,与节间数目无关;突变体的分枝数、荚数、粒数、叶柄长度及夹角、百粒重等产量性状均显著或极显著低于野生型。与野生型相比,突变体叶片叶绿素相对含量和木质素的含量显著高于野生型。本研究结果为控制突变相关基因的定位、图位克隆和功能分析以及育种利用提供了优良种质和理论依据。
Mutant is an important material for gene discovery and function research as well as crop improvement in plants. In this study,we reported the characterization of an ideal plant-architecture mutant( it1) from our EMS mutant population derived from the soybean cv. Zhongpin661( Zp661). Phenotypic analysis revealed that the it1 mutant,compared with the wild type plant,showed more compacting plant architecture,eg,shorter internodes,and darker green leaves. And also the mutant showed a significant decrease in plant height by 30% compared to that of the parental line Zp661,but no significant difference in internode number,indicating that lower plant height for it1 was mainly caused by shortened internode length. Meanwhile,numbers of agronomic traits of the it1 mutant including number of branches,number of pods,petiole length and angle,100-seed weight and yield per plant all displayed a significant or very extremely significant decline than the wild parent. In addition,the physiological characteristics were determined under normal growth conditions in the field,and it1 displayed significantly higher chlorophyll content and cellulose-lignin component than the wild type in seed-filling period. Therefore,the identification of the it1 mutant provides excellent material for mapping,cloning and function research of plant architecture-related genes and genetic improvement in soybean.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2016年第3期523-528,共6页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31271753)
农业部保种课题
中国农业科学院科技创新工程
关键词
大豆
EMS
株型
突变体
Soybean
EMS
mutant
genetic analysis