桥粒芯胶蛋白-1与头颈部鳞状细胞癌发生及预后相关性的研究

Desmocollin-1 significant correlated with the tumorigenesis and prognosis of HNSCC

目的:本研究拟探讨桥粒芯胶蛋白-1与头颈部鳞状细胞癌发生及预后的相关性。方法:本研究通过对NCBI数据库GEO获取的基因芯片数据进行统计学分析,并且采用头颈部鳞状细胞癌组织芯片予以验证。免疫组织化学的方法检测了组织芯片中芯胶蛋白-1的表达,并采用统计学方法分析了芯胶蛋白-1的表达与头颈部鳞状细胞癌发生、分化、预后等的相关性。结果:对GEO获取的芯片数据分析发现芯胶蛋白-1在头颈部鳞状细胞癌中表达增高,且表达增高的患者预后较差。组织芯片的验证实验显示结果与GEO数据库获取数据分析结果基本一致,在头颈部鳞状细胞癌中DSC1的表达显著高于正常组织,且在低分化头颈部鳞状细胞癌中表达高于高分化鳞状细胞癌。结论:桥粒芯胶蛋白-1的增高可促进头颈部鳞状细胞癌的发生,且与肿瘤分化相关。此外,其表达增高提示患者预后不良。

Objective：To explore the correlation between the expression of desmocollin-1and the tumorigenesis and prognosis of HNSCC.Method：Five datasets of HNSCC from the GEO were analyzed.A tumor tissue microassay was chosen for further test.The expression of DSC1 of TMA was detected by immunohistochemical staining.Result：The expression of DSC1 was significantly increased in HNSCC.Meanwhile,the expression of DSC1 was much higher in poor-differentiated tumor than the well-differentiated tumor in HNSCC.What＇s more,the HNSCC patients with lower expression of DSC1 had better outcomes.Conclusion：The results were according with the results of statistical analysis with the bioinformatics data from GEO,indicating that DSC1 significant correlated with the tumorigenesis and prognosis of HNSCC.