油茶粕及其中药复方抗菌及去垢作用研究

Detergent and Antibacterial Effect of Camellia Oleifera Meal and Related TCM Prescription

目的:比较研究油茶粕与油茶粕中药复方的抗菌作用和去垢作用。方法:1采用牛津杯法检测含生药质量浓度为0.5 kg·L^(-1)的油茶粕和油茶粕中药复方提取液对伤寒沙门菌、大肠埃希菌、表皮葡萄球菌、金黄色葡萄球菌、铜绿假单胞菌、痢疾杆菌和乙型溶血性链球菌抑菌环直径;同时采用试管稀释法检测其对以上各供试菌的最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimal bactericidal conceutration,MBC);2采用国标GB9985—2000方法测定含生药质量浓度分别为0.117 kg·L^(-1)、0.235 kg·L^(-1)、0.47 kg·L^(-1)的油茶粕和油茶粕中药复方提取液对不锈钢片上污垢的去垢能力。结果:1含生药质量浓度为0.5 kg·L^(-1)的油茶粕中药复方提取液对金黄色葡萄球菌、乙型溶血性链球菌的抑菌环直径明显大于相应浓度油茶粕提取液;油茶粕中药复方提取液对伤寒沙门菌、表皮葡萄球菌、金黄色葡萄球菌、痢疾杆菌、乙型溶血性链球菌的MIC值均低于油茶粕,对表皮葡萄球菌、金黄色葡萄球菌、乙型溶血性链球菌的MBC值均低于油茶粕;2油茶粕中药复方提取液的去垢能力明显高于相应浓度油茶粕提取液,以含生药质量浓度为0.117 kg·L^(-1)时的差异最明显。结论:油茶粕中药复方对伤寒沙门菌、表皮葡萄球菌、金黄色葡萄球菌、乙型溶血性链球菌的抑菌与和杀菌作用优于油茶粕;油茶粕中药复方的去垢能力明显优于油茶粕。

Objective： To study the detergent and antibacterial effect of Camellia oleifera meal and related traditional Chinese medicine prescription. Methods： 1 Oxford cup method was applied to detect the concentration of 0. 5 kg·L^（-1）crude drug of Camellia oleifera meal and related Chinese medicine prescription extract to know the inhibitory zone diameters of Salmonella typhi,Escherichia coli,Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa bacteria,Shigella and beta-hemolytic streptococcus. The minimum inhibitory concentration（ MIC） and minimum bactericidal concentration（ MBC） were also detected by using tube dilution method; 2 By using GB GB9985-2000 method the detergent power of crude drug of Camellia oleifera meal and related Chinese medicine prescription extract in concentrations of 0. 117 kg·L^（-1）crude drug,0. 235 kg·L^（-1）crude drug,0. 47 kg·L^（-1）crude drug of the both were determined. Results： 1 the inhibitory zone diameters of traditional Chinese medicine prescription extracts with concentration of 0. 5 kg·L^（-1）crude drug working on Staphylococcus aureus and beta-hemolytic streptococcus were significantly larger than that of Camellia oleifera meal in the corresponding concentration. The MIC values of the extracts on Salmonella typhi,Staphylococcus epidermidis,Staphylococcus aureus,Shigella,and beta-hemolytic streptococci were lower than that of Camellia oleifera meal and the MBC values of extracts on Staphylococcus epidermidis,Staphylococcus aureus and beta hemolytic were lower than that of Camellia oleifera meal. 2 The detergent power of the related Traditional Chinese medicine prescription extracts was significantly higher than that of the Camellia oleifera meal extract,with the concentration of 0. 117 kg·L^（-1）crude drug being the most significantly different. Conclusion： The antibacterial role of the Traditional Chinese medicine prescription extracts including Camellia oleifera meal are better than that of Camellia oleifera meal working on Salmonella typhi,Staphylococcus epidermidis,Staphylococcus aureus and beta-hemolytic streptococcus detergent and the detergent power is also superior to the latter.