HPLC法测定水翁花中熊果酸的含量

HPLC Determination of Ursolic Acid in Cleistocalyx Operculatus Flower

目的:采用HPLC法测定水翁花中的熊果酸含量。方法:采用高效液相色谱法(HPLC),以Diamonsil-C18(250mm×4.6mm,5μm)为色谱柱,以乙腈-0.05%磷酸(60∶40)为流动相进行梯度洗脱,检测波长254nm,流速1.0mL/min,柱温25℃。结果:采用HPLC法测定了8批药材熊果酸含量,熊果酸浓度为5~100μg/mL时线性良好。结论:该方法准确、快速、高效,可作为水翁花药材的质量控制。

Objective：To accurately determine ursolic acid content in Cleistocalyx operculatus flower.Methods：The HPLC analysis was carried out in Diamonsil-C18（5μm,250×4.6mm）with a mobile phase of acetonitrile-0.05% phosphoric by gradient.The detection wavelength was set at 254nm;the flow rate was 1.0mL·min^（-1）;and the colum temperarure was kept at 25℃.Results：8batches of the herb samples were analyzed by HPLC to and content of ursolic acid were accurately determined.Conclusion：The method was precise,fast and effective for comprehensive evaluation of quality of the Cleistocalyx operculatus flower.