瘦素受体基因多态性与中国大城市汉族老年人代谢综合征相关性研究

Association of leptin receptor gene polymorphrism with metabolic syndrome in older Han adults from major cities in China

目的研究瘦素受体基因rs1137100（Lys109Arg）和rs1137101（Gln223Arg）多态性位点（SNP）与中国大城市汉族老年人代谢综合征（MS）的相关性。方法根据国际联合暂定声明（JIS）2009年的MS定义，从2002年营养与健康状况调查对象中选取15个省／直辖市18个大城市60岁以上汉族老年人，分为MS组1846人和正常对照组236人，男性701人，女性1381人，共2082人。检测所有研究对象的血浆瘦素和胰岛素，采用Taqman方法检测所有研究对象的瘦素受体基因rs1137100和rs1137101位点的基因型，分析各SNP位点基因型与MS的相关性。结果MS组BMI、腰围、血糖、血压、甘油三酯（TG）、总胆固醇（TC）、胰岛素、胰岛素抵抗指数（HOMA—IR）、瘦素均显著高于正常对照组，高密度脂蛋白胆固醇（HDL—C）显著低于正常对照组。rs1137100和rs1137101位点GG、AA、GA基因型以及A等位基因频率在两组间分布相似。两个位点AA基因型的SBP和DBP均较高，AA及GA＋AA基因型的HDL-C均较低。在rs1137100位点上，与GG型研究对象相比，GA型、AA型研究对象发生MS的风险相似，OR值分别为1．23（95％c，0．90～1．67）和2．23（95％C10．83～6．44）；在rs1137101位点上，与GG型研究对象相比，GA型、AA型研究对象发生MetS的风险相似，OR值分别为1．15（95％C10．74～1．79）和1．97（95％C10．37～10．42）。结论瘦素受体基因rs1137100和rs1137101多态性与中国大城市汉族老年人代谢综合征没有相关性，可能与高血压或血脂异常相关。

Objective To investigate the relationship of leptin receptor gene rs1137100 and rs1137101 single nucleotide polymorphrism（ SNP） with metabolic syndrome（ MS） in older Han adults from major cities in China. Methods A total of 2082 older Han adults were selected from 18 major cities including 15 provinces / municipalities of China National Nutrition and Health Survey in 2002. According to the MS definition proposed by Joint Interim Statement（ JIS）,the subjects were divided into MS and control groups. Plasma leptin and insulin levels were measured. The genotypes of rs1137100 and rs1137101 were detected by Taqman method. Association of genotypes of leptin receptor gene SNPs with MS was investigated. Results The MS group showed higher body mass index（ BMI）,waist circumference,fasting serum glucose,systolic blood pressure（ SBP）and diastolic blood pressure（ DBP）,triglycerides（ TG）,serum total cholesterol（ TC）,insulin,homeostasis model of assessment for insulin resistence index（ HOMA-IR） and leptin levels than those of control individuals,while the high density lipoprotein cholesterol（ HDL-c） was significantly lower than the control group. The GG、AA、GA genotypes distribution and the A allele frequency of rs1137100 and rs1137101 were similar between the two groups. The DBP and SBP level were obviously higher in AA genotype. The HDLc concentration was significantly lower in AA and GA ＋ AA genotype. The AA and GA genotypes carriers in rs1137100 had similar risk for MS when comparing with the GG genotypes,and the OR values were 1. 23（ 95% CI 0. 90-1. 67） and 2. 23（ 95% CI 0. 83-6. 44）,respectively. The AA and GA genotypes carriers in rs1137101 had similar risk for MS when comparing with the GG genotypes,and the OR values were 1. 23（ 95% CI 0. 90-1. 67） and 2. 23（ 95% CI 0. 83-6. 44）,respectively. Conclusion Leptin receptor genes rs1137100 and rs1137101 are not associated with pathogenesis of MS in older Han adults,but it may relate with hypertension or lipid abnormality.