流式细胞术在肿瘤诊断中的应用

The application of flow cytometry in the diagnosis of tumor

肿瘤疾病的早期往往缺乏客观明确的诊断指标，给治疗工作带来很大困扰。如何在癌变的早期发现一些有价值的标志物或者特征性变化，是医学专家们亟需解决的重点难题。随着流式细胞术（FCM）的不断进步，FCM在肿瘤的癌前病变筛查、早期癌变的检出、化疗指导以及预后评估等各个方面发挥着越来越重要的作用。在个体化医疗及精准医疗的时代背景下，FCM在肿瘤标志物的检测、肿瘤干细胞（CSCs）分离筛选、循环肿瘤细胞（CTCs）的分离鉴定等方面的应用尤其值得期待。

In the early stage of tumor, the diagnosis index of objective and definite is often deficient, which brings great trouble to the treatment How to find some valuable markers or characteristic changes in the early stage of cancer is the key problem to be solved by the medical experts. With the development of flow cytometry （ FCM）, it is playing a more and more important role in precancerous lesion screening, early detection of cancer, chemotherapy guidance and prognosis assessment In personalized medicine and precise medical background, the application of FCM in tumor marker detection, cancer stem cells separation screening, isolation and identification of circulating tumor cells is especially worth looking forward to. （ Chin J Lab Med, 2016, 39： 336-339）