氢生理盐水对对乙酰氨基酚致小鼠急性肝损伤的保护作用

Protective effect of hydrogen-rich saline against acetaminophen-induced liver injury in mice

目的观察氢生理盐水对对乙酰氨基酚致小鼠急性肝损伤的保护作用。方法乙酰氨基酚500mg/kg腹腔内注射诱发小鼠急性肝损伤后,通过测定血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)及肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平,肝匀浆丙二酫(MDA)和还原型谷胱甘肽(GSH)含量,观察肝脏病理学改变,评价氢生理盐水对对乙酰氨基酚致小鼠急性肝损伤的保护作用。结果氢生理盐水能降低对乙酰氨基酚致小鼠急性肝损伤的血浆ALT、AST水平,显著抑制炎症因子TNF-α、IL-6的生成和脂质过氧化反应产物MDA的含量,并增加GSH储备,有效减轻肝细胞坏死的严重程度。结论氢生理盐水对对乙酰氨基酚致小鼠急性肝损伤具有明显的的保护作用。

Objective To investigate the protective effect of hydrogen-rich saline against acetaminophen-induced liver injury in mice. Methods Mice liver injury was induced by peritoneal injection acetaminophen saline solution at a dose of 500 mg/kg. The levels of plasma alanine aminotransferase（ALT） , asparate aminotransferase（AST） , tumor necrosis factor alpha（TNF-α） , interleukin- 6（IL-6） and the contents of malondialdehyde （MDA）, glutathione （CSH） in the liver tissue were measured, and the pathological change of liver tissue were observed to evaluate the effects of hydrogen-rich saline（HS） on liver injury induced by acetaminophen in mice. Results In acetaminophen-induced liver injury in mice, HS can significantly reduce the levels of plasma ALT, AST; inhibit the generation of inflammatory factors TNF-α and IL-6 ; decrease the content of the MDA of the lipid peroxidation products and increase the reservation of GSH ; diminish the degree of hepatocyte necrosis. Conclusion The hydrogen-rich saline has a protective role against acetaminophen-indueed liver injury in mice.