肺结核患者治疗前后外周血中IL-10、IL-22、IL-23水平变化及其临床意义

Levels of IL-10, IL-22, and IL-23 in peripheral blood of patients with pulmonary tuberculosis before and after treatment and their clinical significance

目的分析肺结核患者治疗前后外周血中IL-10、IL-22、IL-23表达水平变化及其临床意义。方法收集本院2014年10月至2015年5月收治的活动性肺结核患者49例，选择健康体检者30例为对照组。ELISA法检测肺结核患者治疗前、治疗6个月后以及对照组外周血血清中IL-10、IL-22、IL-23表达水平，并分析其相关陡。结果ELISA结果显示，肺结核患者治疗前外周血血清IL-10表达水平为（24．15±13．21）pg／ml，明显高于对照组[（14．91±8．23）pg／m1]，P〈0．05，经抗结核常规治疗6个月后，血清IL-10表达水平为（17．11±7．59）pg／ml，明显低于治疗前（P〈0．05），但仍高于对照组。肺结核患者治疗前外周血血清IL-22、IL-23表达水平分别为（20．56±7．41）pg／ml、（21．12±2．68）pg／ml，明显低于对照组[（53．78±8．22）pg／ml、（36．42±2．16）pg/ml，P〈0．05]，经抗结核常规治疗6个月后，血清IL-22、IL-23表达水平分别为（38．95士6．35）pg／ml、（29．89±3．74）pg／ml，明显高于治疗前（P〈0．05），但仍明显低于对照组（P〈0．05）。Pearson相关分析结果表明，血清IL-22、IL-23呈正相关性。结论肺结核患者外周血中IL-10、IL-22、IL-23在治疗前后表达水平发生变化，可能与机体结核免疫有关，有望作为评价肺结核疗效的参考指标。

Objective To study the levels of IL-10, IL-22, and IL-23 in peripheral blood of patients with pulmonary tuberculosis before and after treatment and their clinical significance. Methods 49 patients with pulmonary tuberculosis treated at our hospital from October, 2014 to May, 2015 were enrolled into this study. And 30 healthy volunteers were selected as a control group. ELISA were used to measure the levels of IL-10, IL- 22, and IL-23 in peripheral blood of the patients and the control group before and 6 months after the treatment. The correlation was analyzed by pearson correlation analysis. Results The serum levels of IL-10, IL-22, and IL- 23 were （24.15±13.21） pg/ml, （20.56±7.41） pg/ml, and （21.12±2.68） pg/ml in the patients and were （14.9±8.23） pg/ml, （53.78±8.22） pg/ml, and （36.42±2.16） pg/ml in the control group before the treatment （P〈0.05） and were （17.11±7.59） pg/ml, （38.95±6.35） pg/ml, and （29.89±3.74） pg/ml in the patients after the treatment （P〈0.05）. Conclusions IL-10, IL-22, and IL-23 may play a role in immune response in anti-tuberculosis therapy by modulating the serum levels in peripheral blood and could be indicators of clinical efficacy.