根癌农杆菌介导GUS基因转化玉米芽尖生长点的研究

Study on Agrobacterium tumefaciens–mediated transformation of GUS gene into maize shoot apical growing point

本试验以玉米(Zea mays)杂交种‘郑单958’的芽尖生长点作为农杆菌侵染受体,以β-葡萄糖醛酸苷酶基因(GUS)作为转化基因,吸水链霉菌草丁膦乙酰转移酶基因(bar)作为筛选标记基因,通过GUS颜色反应,分别从侵染液浓度、侵染时间和真空处理时间三个方面分析了影响GUS基因转化率的因素,初步得出结论:当侵染液浓度(OD600)为0.6、侵染时间2 h、真空处理时间为10 min时,选用杂交种‘郑单958’作为受体材料将会达到较高的遗传转化率。

Using shoot apical growing point of maize hybrid ‘zhengdan958＇ seeds as explants, several factors affecting the Agrobacterium tumefaciens–mediated genetic transformation were studied with β-glucuronidase（GUS） as transformation gene and Streptomyces hygroscopicus phosphinothricin acetyltransferase（bar） as screening gene. The results indicate that good transformation efficiency can be obtained when the concentration of bacterial OD_（600） is 0.6, the co-cultivation time is 2 h, the vacuum infiltration time is 10 min and the receptor is ‘zhengdan958＇.