发芽糙米膳食纤维制备及其降血脂活性研究

Study on dietary fiber prepared from germinated brown rice and its activity of anti-hyperlipidemia

发芽糙米(PGBR)能显著改善高脂血症,其膳食纤维(DF)可能是主要活性成分之一。采用酶法制备了发芽糙米膳食纤维(PGBR–DF),测定了PGBR–DF的组成,并研究了PGBR–DF的功能特性及降血脂活性。PGBR–DF得率为61.5%,可溶性DF含量为26.4%。与米糠DF相比,PGBR–DF持水(5.96 g/g)、持油力(5.47 g/g)更高;吸附胆酸钠能力更强,特别是可溶性PGBR–DF胆汁酸钠吸附量达0.034 g/g,普通米糠DF仅为0.018 g/g。PGBR-DF能显著改善高脂小鼠的胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)指标(ρ<0.05);特别是高剂量(10%)PGBR–DF可使高脂鼠的TC、TG指标恢复正常,与正常对照无显著差异(ρ<0.05)。

Pre–germinated brown rice（PGBR） could significantly improve hyperlipidemia,and the dietary fiber（DF） was suggested as one of the main active ingredients. The dietary fiber of pre–germinated brown rice（PGBR–DF） was prepared by enzymatic method,the composition of PGBR–DF was determined,the functionalities and anti–hypolipidemic activity of PGBR–DF were studied. The yield of PGBR–DF was 61.5%,the content of soluble DF was 26.4%. Compared with the rice DF,water holding（5.96 g/g）,and oil holding capacity（5.47 g/g） of PGBR–DF were higher and adsorption of sodium cholate ability was stronger,especially the amount of bile acid sodium adsorbed by soluble PGBR–DF was 0.034 g/g,and that for ordinary rice bran DF was only 0.018g/g. PGBR–DF could improve TC,TG,HDL–C and LDL–C indicators of high fat rats significantly（ρ0.05）. There was no significant difference（ρ0.05） between the H group（10%） and normal control for TC and TG.