HPLC法测定疱疹康喷雾剂中6种成分

Determination of six constituents in Paozhenkang Spray by HPLC

目的建立HPLC法测定疱疹康喷雾剂（大黄、黄连、白芷等）中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、盐酸小檗碱的含有量。方法盐酸小檗碱的分析采用Venusil XBP C18（L）色谱柱（4.6 mm×250 mm,5μm）;流动相为乙腈-水-冰乙酸-三乙胺（28∶70∶1∶1）;检测波长265 nm;柱温25℃;体积流量1 m L/min。其他5种成分的分析采用Venusil XBP C18（L）色谱柱（4.6 mm×250 mm,5μm）;流动相为甲醇-0.1%磷酸溶液（75∶25）;检测波长254 nm;柱温25℃;体积流量1 m L/min。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、盐酸小檗碱分别在0.017 9~0.358μg（r=0.999 9）、0.018 2~0.364μg（r=0.999 8）、0.018 9~0.338μg（r=0.999 9）、0.008 0~0.160μg（r=1）、0.008 9~0.177μg（r=0.999 9）、0.147~1.470μg（r=0.999 7）范围内线性关系良好,平均回收率分别为101.3%（RSD=1.0%）、102.8%（RSD=1.5%）、101.2%（RSD=1.9%）、102.7%（RSD=1.3%）、100.2%（RSD=1.9%）、101.3%（RSD=2.0%）。结论该方法灵敏、简便、准确、重复性好,可用于疱疹康喷雾剂的质量控制。

AIM To establish an HPLC method for determining the contents of aloe-emodin,rhein,emodin,chrysophanol,physcion and berberine hydrochloride in Paozhenkang Spray（ Rheum palmatum,Coptis chinensis,Angelica dahurica,etc.）. METHODS The analysis of berberine hydrochloride was conducted on Venusil XBP C18（ L）column（ 4. 6 mm × 250 mm,5 μm） with its temperature maintained at 25 ℃,a mobile phase of acetonitrile-water-acetic acid-triethylamine（ 28 ∶ 70 ∶ 1 ∶ 1）,265 nm for detection wavelength,and flow rate of1 m L / min. The analyses of another five constituents were performed on Venusil XBP C18（ L）column（ 4. 6 mm ×250 mm,5 μm） and a mobile phase comprising of methanol-0. 1% phosphoric acid solution in ratio 75 ∶ 25.The other conditions were 254 nm detection for wavelength,25 ℃ for column temperature,and 1 m L / min for flow rate. RESULTS Aloe-emodin,rhein,emodin,chrysophanol,physcion and berberine hydrochloride showed good linear relationships within the ranges of 0. 017 9-0. 358 μg（ r = 0. 999 9）,0. 018 2-0. 364 μg（ r = 0. 999 8）,0. 018 9-0. 338 μg（ r = 0. 999 9）,0. 008 0-0. 160 μg（ r = 1）,0. 008 9-0. 177 μg（ r = 0. 999 9） and0. 147-1. 470 μg（ r = 0. 999 7）,whose average recoveries were 101. 3%,102. 8%,101. 2%,102. 7%,100. 2% and 101. 3% with the RSDs of 1. 0%,1. 5%,1. 9%,1. 3%,1. 9% and 2. 0%, respectively.CONCLUSION This sensitive,accurate,simple and reproducible method qualifies in the quality control of Paozhenkang Spray.