UPLC/ESI-Q-TOF MS法分析鲜地黄、生地黄、熟地黄的化学成分

Analysis of chemical constituents in fresh,dried and prepared Rehmanniae Radix by UPLC / ESI-Q-TOF MS

目的建立超高效液相色谱-电喷雾/四极杆飞行时间串联质谱联用(UPLC/ESI-Q-TOF MS)法分析鲜地黄、生地黄、熟地黄中的化学成分。方法分析采用Agilent Zorbax SB-C18色谱柱(4.6 mm×100 mm,1.8μm);流动相为0.1%甲酸溶液-甲醇,梯度洗脱;柱温40℃;体积流量0.3 m L/min;进样量2μL。结果共鉴定了26个化学成分,包括12个环烯醚萜苷、8个苯乙醇苷、3个核苷、2个有机酸、1个紫罗兰酮。梓醇、益母草苷、毛蕊花糖苷是鲜地黄和生地黄中的主要成分,而在熟地黄中含有量较低;地黄苷D在3种炮制品中的含有量均较高,比较稳定。结论该方法可为控制地黄质量提供科学依据。

AIM To establish an ultra performance liquid chromatography / electrospray ionization quadrupole time-of-flight tandem mass spectrometry（ UPLC / ESI-Q-TOF MS） method for analyzing the chemical constituents in fresh,dried and prepared Rehmanniae Radix. METHODS The analysis was carried out on an Agilent ZORBAX SB-C18column（ 4. 6 mm × 100 mm,1. 8 μm）,mobile phase was 0. 1% formic acid solution-methanol with gradient elution,column temperature was maintained at 40 ℃,flow rate was 0. 3 m L / min,and injection volume was2 μL. RESULTS Total twenty-six chemical constituents were identified,including twelve iridoid glycosides,eight phenylethanoid glycosides,three nucleosides,two organic acids,and one ionone. Catalpol,leonuride and verbascoside were the main constituents in fresh and dried Radix Rehmannia,whose contents were low in prepared Rehmanniae Radix. The content of rehmannioside D was high in three processed products,which was relatively stable. CONCLUSION This method can provide the scientific evidence for controlling the quality of Rehmanniae Radix.