HnRNPL蛋白在人前列腺癌组织中的表达及其临床意义

Expression of hnRNP L protein in human prostate cancer tissues and its clinical significance

目的 研究hnRNP L蛋白在前列腺癌中的表达及临床意义.方法 收集我院2011年1月至2013年5月期间行前列腺癌根治术后标本60例,良性前列腺增生（BPH）组织标本40例.采用Western blot及免疫组化法检测hnRNP L蛋白在前列腺癌及良性前列腺增生组织中的表达,并分析其表达与临床病理参数之间的关系.结果 Westem blot结果显示hnRNP L蛋白在前列腺癌组织中的表达明显高于良性前列腺增生组织（P＜0.05）.免疫组化结果表明hnRNP L蛋白阳性表达于细胞核,其在前列腺癌及前列腺增生组织中的阳性表达率分别为66.7％、17.5％,差异具有统计学意义（P＜0.05）.此外,hnRNPL在前列腺癌组织中的表达与T分期、临床分期以及Gleason评分密切相关（P＜0.05）.结论 hnRNPL高表达于前列腺癌组织,与前列腺癌的发生发展存在相关关系,有可能作为前列腺癌早期诊断标志物和治疗靶点.

Objective To Investigate the expression of hnRNP L in human prostate cancer and explore its clinical significance. Methods Sixty cases of prostate cancer and 40 cases of benign prostate hyperplasias in our hospital during January, 2011 and May, 2013 were enrolled in this study. The expression of hnRNP L protein in prostate cancer tissues and benign prostate hyperplasias tissues was detected by Western blotting and immunohistochemistry （IHC）, respectively. Meanwhile, the correlation between hnRNP L expression and clinical pathological characters was analyzed. Results Western blotting analysis showed that hnRNP L expression in prostate cancer tissues was significantly higher than that in in benign prostate hyperplasias tissues （P〈0.05）. IHC analysis demonstrated that positive expression of hnRNP L was located in nuclear of cells, and the positive rates of hnRNP L in prostate cancer and benign preostate hyperplasias were 66.7% and 17.5%, respectively, with a significant difference （P〈0.05）, Moreover, hnRNP L expression was positively correlated with T stage, clinical stage and Gleason score in prostate cancer （P〈0.05）. Conclusion hnRNP L is highly expressed in prostate cancer tissues, and correlated with tumor occurrence and development, which may be served as effective molecular marker or therapeutic target in prostate cancer.