丹参多糖对急性肝损伤小鼠肝窦内皮细胞窗孔的影响

Effect of Salvia miltiorrhiza polysaccharides on sinusoidal endothelial cell fenestrae in mice with acute liver injury

目的:探讨丹参多糖(SMPS)对急性肝损伤小鼠肝窦内皮细胞(LSEC)窗孔的影响。方法:尾静脉注射脂多糖(LPS)诱导小鼠急性肝损伤模型,检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、一氧化氮(NO)的水平,比较肝组织中小窝蛋白-1(Car-1)的表达情况及LSEC窗孔的改变情况。结果:SMPS能降低血清中AST、ALT、NO的含量,减少Car-1的表达,增加LSEC的数目,并其使窗孔孔径增大。结论:SMPS通过开放LSEC窗孔,改善肝窦微循环,达到保护肝脏的作用。

Objective：To investigate the effect of Salvia miltiorrhiza polysaccharides（SMPS） on sinusoidal endothelial cell（LSEC） fenestrae in mice with acute liver injury.Methods：Mice models with acute liver injury were induced by lipopolysaccharide（LPS） through tail vein injection.The levels of serum aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,and nitric oxide（NO） were determined.The changes of caveolin-1（Cav-1） expression and LSEC fenestrae in liver tissues were compared.Results：SMPS could decrease the content of serum AST,ALT and NO,reduce the expression of Cav-1,and increase the number of LSEC and the diameters of fenestrae.Conclusion：SMPS can improve sinusoidal microcirculation by opening LSEC fenestrae to protect liver.