复合氨基酸联合维生素B_6救治四氧化二氮吸入中毒小鼠的实验研究

Compound amino acid and vitamin B_6 for treatment of inhaling nitrogen tetroxide induced acute pulmonary edema in mice

目的探讨复合氨基酸联合维生素B_6治疗四氧化二氮吸入中毒肺水肿小鼠的疗效。方法实验动物为ICR小鼠24只,体重23-25 g,随机分为对照组、中毒组和治疗组等三组（8只）。将动物置于120 L染毒柜制作N_2O_4吸入中毒致肺损伤动物模型,于48 h采用眼球摘除法取血,分离血清-70℃保存,ELISA法测定ANP、SP-D含量。结果对照组小鼠血清ANP和SP-D含量均值分别为[（221.74±30.82）mg/L、（24.09±2.79）mg/L];中毒组分别为[（313.46±31.58）mg/L、（20.40±4.27）mg/L];药物干预组分别为（262.03±26.50、23.20±2.84）。中毒组与对照组之间ANP和SP-D水平差异有统计学意义（t_（ANP）=7.85,t_（SP-D）=6.89,P〈0.05）,治疗组与中毒组之间ANP和SP-D水平差异有统计学意义（t_（ANP）=4.52,t_（SP-D）=3.67,P〈0.05）。结论采用丰诺安联用维生素B_6能明显改善N2O4吸入中毒动物血清ANP和SP-D代谢水平,促进病情转归,对于救治此类氮氧化物中毒患者具有一定参考价值。

Objective To explore the diagnosis and treatment compound amino acid and vitamin B6 nitrogen tetroxide inhalation poisoning the curative effect of pulmonary edema.Methods ICR mice 24, weight 23-25 g （n =8）,were randomly divided into three groups：control group,poisoned group,and treatment group.The mice were put in the 120 L exposed cabinet to inhalate nitrogen oxides as environmental exposure mode.Bloods were collected by the eye-excised method at 48 h time point,the serum was separated by -70℃ preservation,and the ELISA was used to detect the contents of ANP and SP-D.Results Serum ANP and SP-D were （221.74 ±30.82）mg/L and （24.09 ±2.79）mg/L in control group,（313.46 ± 31.58）mg/Land （20.40 ±4.27）mg/L in the poisoned group,and （262.03 ±26.50）mg/L and （23.20 ± 2.84）mg/L in the treatment group,respectively.We used SPSS20.0 software to perform single factor analysis of variance,and P 〈0.05 was set as statistical significance.The difference was statistically significant between the poisoned group and the control group in the ANP and SP-D levels （tANP =7.85, tSP-D =6.89,P 〈0.05 ）,and the same to that between the poisoned group and the treatment group in the ANP and SP-D levels （tANP =4.52,tSP-D =3.67,P 〈0.05 ）,but no significant difference existed in the level of TGF-β1.Conclusions It leads to impairment of pulmonary edema in short time exposure of nitrogen oxides.To detect the level of ANP and SP-D could be helpful to study the pulmonary edema. Compound amino acid and vitamin B6 could obviously improve the level of serum ANP and SP-D in the animals poisoned by N2O4 ,and disease outcome,which have a certain reference value in the treatment of such patients.