20AA复方氨基酸联用大剂量维生素B6治疗溴敌隆中毒致凝血能障碍大鼠的实验研究

Effects of new therapy of 20AA compound amino acid plus high dose of vitamin B6 on trauma-induced coagulopathy

目的：观察20AA 复方氨基酸（丰诺安）＋大剂量维生素 B6对溴敌隆中毒致凝血功能障碍动物模型凝血功能的影响。方法实验动物为雄性SD大鼠，随机分为正常组，对照组和新疗法治疗组，每组8只，以4 mg／kg 溴敌隆稀释液灌胃制作大鼠溴敌隆急性中毒致凝血功能障碍模型，颈静脉插管输注复方氨基酸注射液丰诺安＋大剂量维生素 B6，对照组输注生理盐水，72 h 后，检测大鼠的各项凝血功能指标变化凝血酶原时间（PT）、凝血酶时间（TT），显著性分析采用双尾 t 检验。结果将治疗后新疗法治疗组与对照组比较，凝血功能试验结果：凝血酶原时间（PT）（16．3±5．20）s 显著低于对照组（24．51±4．70）s，t ＝3．31，P ＜0．05；凝血酶时间（TT）（21．04±5．66）s 显著低于对照组（26．59±3．40）s，t ＝2．38，P ＜0．05而活化部分凝血活酶时间（APTT）和纤维蛋白原（FIB）与对照组无明显差异。纤维蛋白原（FIB）（2．96±0．35）g／L 与对照组（2．68±0．63）g／L，t ＝0．67，P ＜0．05，无明显差异。活化部分凝血酶时间（APTT）部分数据仪器检测不出，无法进行统计学检验。结论丰诺安＋维生素 B6联用能够明显改善急性溴敌隆中毒后大鼠凝血功能。

Objective Establish the experimental animal model of coagulation dysfunction by Bromadiolone poisoning.Observe the effect of a new therapy for injecting Compound Amino Acid （20AA） combined with large doses of vitamin B6 on coagulation dysfunction in animal model. Methods Experimental animal for male SD rats,were randomly divided into normal group,control group and therapy group,with 8 rats in each group.The coagulation dysfunction animal model of Bromadiolone acute poisoning was made with 4 mg/kg Bromadiolone diluents by gastric lavage.The therapy group was jugular vein injected with Compound Amino Acid combined with large doses of vitamin B6 ,and the control group was injected with physiological saline.72 hours later,observe the changes of coagulation indexes of rats.Results The new therapy group compared with the control group after treatment,Prothrombin time （PT）（16.3 ±5.20）s was significantly lower than the control group （24.51 ±4.70）s,t =3.31,P 〈0.05;Thrombin time （TT） （21.04 ±5.66）s was significantly lower than the control group （26.59 ±3.40）s,t =2.38,P 〈0.05.The activated partial thromboplastin time （APTT）and fibrinogen （FIB）had no significant differences between the two groups.Fibrinogen（FIB）（2.96 ±0.35 ）g/L had no significant difference with the control group （2.68 ±0.63）g/L,t =0.67,P 〈0.05.The data of activated partial thromboplastin time（APTT）can not be fully detected by instruments or statistical tested..Conclusion Injecting Compound Amino Acid （20AA） combined with large doses of vitamin B6 can obviously improve the rats′coagulation function of Bromadiolone acute poisoning.