油菜蕾薹期和花期菌核病接种方法比较

Comparison of Methods for Resistance Screening aganist Sclerotinia sclerotiorum in Brassica napus

核盘菌引起的菌核病是油菜重要病害。为建立快速有效的油菜菌核病抗性鉴定方法,在油菜花期采用4种人工接种方法测定了5种油菜资源对菌核病的抗病性,并分析采用不用接种方法测定的病斑大小之间的相关性。这4种方法分别为:离体叶片接种、离体茎段接种(牙签法)、活体茎秆接种(牙签法)、活体叶柄接种(吸头法)。这4种接种方法中,离体叶片接种法与其余3种方法的鉴定结果均没有显著相关性;采用叶柄接种法,接种后11 d和19 d的病斑长度与离体茎段接种3 d和7 d的病斑长度之间的相关性显著(P<0.05),相关系数分别为0.866和0.821,与活体茎秆接种7 d和15 d的病斑长度极显著相关(P<0.01),相关系数分别为0.952和0.987。结果表明移液管介导的叶柄接种是一种便捷、可靠的油菜菌核病抗性鉴定方法。

Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a major disease in oilseed rape.To establish a rapid and effective method for evaluation of resistance in Brassica napus to S. sclerotiorum four artificial inoculation methods and five genotypes of Brassica napus were used in this study. The four methods were detached leaf inoculation（ DLI）,detached stem inoculation with toothpick（ DSI）,stem inoculation with toothpick in vivo（ SIT）,and petiole inoculation with pipette tip in vivo（ PIT）. No significant correlations were detected between detached leaf inoculation and the other three methods.The lesion length of PIT at 11 dpi（ days post-inoculation） and 19 dpi showed significant（ P0.05） correlations with lesions of DSI at 3dpi and 7 dpi（ r= 0.866 and 0.821）,and highly significant（ P0.01） correlations with lesions of SIT at 7 dpi and 15 dpi（ r= 0.952 and 0.987）.These results suggest that the petiole inoculation with pipette tip is a convenient and reliable method for screening the resistance against S.sclerotiorum in B.napus.