烟酸诺氟沙星在松浦镜鲤体内代谢残留规律的研究

A Study on the Metabolic Residue Rule of Norfloxacin Nicotinate in Common Carp

分析烟酸诺氟沙星在松浦镜鲤体内代谢残留规律,准确制定该种药物的休药期,为有效、安全、合理地使用烟酸诺氟沙星提供准确科学的理论依据。选用松浦镜鲤(Cyprinus carpio L.)为实验对象,连续5 d口灌30 mg/(kg·d)烟酸诺氟沙星(norfloxacin nicotinate,NFX)后,分别于给药后1,2,3,4,5,6,7,9,11,13,15,20,25 d采集鲤血浆、肌肉、皮、肝和肾5种组织,通过高效液相色谱法检测其各组织内烟酸诺氟沙星的残留量。烟酸诺氟沙星在鲤体内吸收分布迅速,代谢较为缓慢,各组织分布量由多到少依次为:肾脏、肝胰脏、肌肉、皮肤、血浆,为避免组织内残留过多药物,建议休药期为24 d。

The aim of this study is to study the residual rule of norfloxacin nicotinate in common carp,formulate the withdrawal time of norfloxacin accurately and provide a precision and scientific theory basis for using this medicine reasonably,safely and effectively. In the experiment,common carp（ Cyprinus carpio L.） was taken as the target,the fish were drenched with the medicine 30 mg /（ kg ·d） for 5 days,then their kidney,liver,muscle,skin and blood were collected at the 1st,2nd,3rd,4th,5th,6th,7th,9th,11 th,13th,15 th,20th,25 thday after the medication.The residual of norfloxacin nicotinate in the above 5 kinds of tissues was detected by high performance liquid chromatography（ HPLC）.Norfloxacin nicotinate was absorbed by the tissues rapidly but metabolized slowly.The distribution of the medicine was in the order： kidneylivermuscleskinblood.In order to avoid excessive medicine residues within the tissues,the 24 days of withdrawal time is proposed.