饲料中添加蚯蚓对黄鳝生长及消化酶活力的影响

The Effects of Earthworms on Growth and Digestive Enzyme Activity of Rice Field Eel

为了解蚯蚓对黄鳝生长及消化酶活力的影响,分别用添加0%（对照组）、25%、50%、75%、100%鲜蚯蚓的饲料饲喂黄鳝61 d。结果表明：添加25%蚯蚓的饲料组增重率、特定生长率最大与对照组差异显著;添加50%蚯蚓组肥满度最佳与对照组差异显著;胃蛋白酶活性随蚯蚓添加量增加先降低后升高,肠道中胰蛋白酶活性随蚯蚓添加有一直升高的趋势。胰脂肪酶呈现随蚯蚓添加先升高后下降的趋势。淀粉酶活性,胃、肠中总体上均较低,且胃中淀粉酶活性低于肠中淀粉酶活性,添加25%-50%蚯蚓能显著提高胃、肠中淀粉酶活力。结论：添加适量蚯蚓可促进黄鳝对饲料中蛋白质、脂肪和淀粉的消化,提高黄鳝的生长性能,结合黄鳝生长性能回归分析结果和消化酶活性变化情况,黄鳝饲料中蚯蚓适宜添加量为35%-40%。

In order to understand the effects of earthworms on growth performance of rice field eel（ Monopterus albus）,eels were fed with the food contained 0%（ control group）,25%,50%,75% and 100% of earthworms respectively for 61 days.The results show that： the weight gain rate,specific growth rate of the 25%earthworm group were significantly higher than those of the control group. The fertility of the 50% earthworm group was significantly higher than that of the control group.With the increasing of earthworm addition,there was a rising trend in the activity of proteinase in intestinal,while it decreased at first and then increased in the stomach.The pancreatic lipase in intestinal showed the tendency of increasing at first and then decreasing with the increase of earthworms.Amylase activity was lower in both of stomach and intestinal,and it was higher in intestine than in stomach,by comparison,25%- 50% of earthworm could significantly improve the amylase activity in stomach and intestine.Conclusion： adding earthworms can promote digestion of dietary protein,fat and starch of rice field eel and improve the growth performance of rice field eel.According to the results of growth performance regression analysis and activity of digestive enzymes,35%-40% of earthworm addition in feed of rice field eel is the optimum.