2型糖尿病大鼠六味地黄丸治疗后血脂、血糖以及IL-2和IL-10水平的变化

Changes of Blood Lipids,Blood Sugar,IL-2 and IL-10 Levels of Type 2 Diabetes Rats After Liuwei Dihuang Pill Treatment

目的探讨六味地黄丸治疗2型糖尿病大鼠的疗效。方法构建2型糖尿病、中医分类的阴虚热盛型和气阴两虚型三种的大鼠模型,模型组均采用六味地黄丸干预治疗,正常对照组大鼠采用安慰剂干预,检测四组大鼠干预前后的血糖(Glu)、三酰甘油(TG)、总胆固醇(TC)以及细胞因子IL-2和IL-10的水平。结果正常对照组的Glu、TC和TG均无明显变化。模型组干预治疗后的Glu和TG水平均明显下降(P<0.05),而TC除阴虚热盛组水平下降明显(P<0.05)外,其余各组的变化不明显;模型组IL-2和IL-10均呈明显弱表达,而治疗后两者的升高较为明显(P<0.01)。结论在大鼠模型中,六味地黄丸治疗2型糖尿病的疗效明显,可引起细胞因子IL-2和IL-10水平的高表达,其疗效可能与Th1/Th2平衡相关。

Objective To explore the curative effect of Liuwei Dihuang pill for the treatment of type 2 diabetes mellitus （T2DM） rats. Methods Three kinds of rat models were established, including T2DM model （T2DM group）, Yin-Xu-Re-Sheng model （YXRS group） and Qi-Yin-Liang-Xu model （QYLX group）. Rats of normal control group received placebo intervention, and rats of model groups received Liuwei Dihuang pill intervention. The levels of glucose （Glu）, triglyceride （TO）, total cholesterol （TC）, IL-2 and IL-10 before and after intervention of four groups were detected. Results The levels of Glu, TC and TG of normal control group before and after intervention had no significant change （P 〉0.05）. The levels of Glu and TO of model groups decreased significantly after intervention （P 〈0.05）; After intervention, the TC level decreased significantly in YXRS group （P〈0.05）, and had no significant change in the other two groups （P〉0.05）. Before intervention, the levels of IL-2 and IL-10 of model groups showed obviously low expression, while after intervention, the IL-2 and IL-10 levels increased significantly （P〈0.01）. Conclusions Liuwei Dihuang pill has significant efficacy for T2DM rats, which can lead to high expressions of IL-2 and IL-10. The efficacy may relate to Th1 / Th2 balance.