摘要
对翼蓼块根的体积分数90%乙醇提取物及其不同极性溶剂萃取物的抗氧化活性和抑制MCF-7癌细胞增殖作用进行研究.结果表明:乙酸乙酯层萃取物的总酚和黄酮高达211.99 mg没食子酸/g和21.50 mg槲皮素/g,其DPPH自由基清除能力的EC_(50)值为14.08μg/m L.测定还原能力结果表明乙酸乙酯层萃取物层质量浓度为1 000μg/mL时,吸光度为1.09.乙酸乙酯层萃取物质量浓度为2 mg/mL时,MCF-7癌细胞增殖抑制率达97.15%.
The 90% ethanol extract of Pteroxygonum giraldii root and its fractions of several different polar solvents were used to investigate the antioxidant activities and inhibition ability for MCF-7cancer cell proliferation in this study.The results showed that the total phenolic and flavonoid contents of the ethyl acetate fraction were high up to211.99 mg gallic acid/g and 21.50 mg quercetin/g respectively.The ethyl acetate fraction exhibited strong DPPH free radical scavenging with the EC_（50） of 14.08 μg/m L.Reduction ability of the ethyl acetate fraction showed that the absorbance was 1.09 at 1 000 μg/m L.The ethyl acetate fraction exhibited the 97.15% MCF-7cancer cell proliferation inhibition rate at 2 mg/m L.
出处
《河南科技学院学报（自然科学版）》
2016年第2期53-58,共6页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
河南科技学院大学生创新训练计划
关键词
翼蓼
总酚
总黄酮
DPPH
MCF-7癌细胞
Pteroxygonum giraldii
total phenolic
total flavonoid
DPPH
MCF-7cancer cell
