Pokemon在子宫内膜癌组织中的表达及其与突变型p53的相关性研究

The Expression of Pokemon in Endometrial Carcinoma Tissue and the Correlation with Mutant p53

目的初步探讨Pokemon表达与子宫内膜癌（endometrial carcinoma,EC）临床特征之间的关系,并分析不同类型EC组织中Pokemon表达与p53突变的关系,为寻求基因治疗的有效靶点提供理论基础。方法收集四川大学华西第二医院病理科2012年7月至2014年7月的子宫内膜存档石蜡标本共98例（其中EC 58例,正常内膜组织20例,非典型增生内膜组织20例）,采用免疫组化法检测每例切片中Pokemon蛋白的表达情况,分析其表达水平与临床病理特征的关系,并分析Pokemon和突变型p53两者之间的相关性。结果 Pokemon在正常内膜组织、非典型增生内膜组织、EC组织的阳性率分别为25.00%（5/20）、60.00%（12/20）、93.10%（54/58）,表达程度逐渐增强（P均〈0.05）;Pokemon在Ⅱ型EC组织的阳性率97.14%（34/35）高于Ⅰ型的阳性率86.96%（20/23）（P=0.018）;Pokemon在Ⅲ~Ⅳ期、Ⅱ型及Ki-67增殖指数≥50的内膜癌组织中表达程度较高（P=0.012、0.023、0.029）;Ⅱ型EC组织中,Pokemon和突变型p53的秩和相关系数rs=0.669（P=0.000）。结论 Pokemon的高表达引起突变型p53表达上调,可能是Ⅱ型EC的致癌模式之一。

Objective To detect the expression of Pokemon in endometrial carcinoma（EC）,to provide preliminary theoretical basis for clarifying pathogenesis and searching for effective targets.Methods Ninety-eight cases of endometrial tissue paraffin specimens form July 2012 to July 2014 in West China Second University Hospital,Sichuan University,were collected,including：EC group,consisting of adenocarcinoma 23 cases,adenosquamous 12 cases,serous 3cases,mucinous 11 cases and clear cell 9cases,and control group,consisting of atypical hyperplasia endometrium 20 cases and normal endometrium 20cases（secretory 10 cases,hyperplasia 10cases）.Immunohistochemistrywas used to detect the expression of Pokemonin each section,analyzing the correlation of Pokemon expression with clinicopathologic characteristics and p53 expression.Results The positive rate of Pokemon in normal endometrium was 25%（5/20）,significantly lower than that in atypical hyperplasia endometrium（60.0%,12/20）and EC（93.1%,54/58）（P〈0.05）;the rate in type Ⅱ was 97.12%（34/35）,significantly higher than that in type Ⅰ（86.96%,20/23）（P=0.018）.The positive rate of Pokemon in Ⅲ-Ⅳstage,typeⅡ and Ki-67≥50EC tissue was much higher（P=0.012,0.023,0.029）.In type Ⅱ EC tissue,the correlation index between Pokemon and p53 is 0.669（P=0.000）.Conclusion The over expression of Pokemon upregulates the expression of mutant p53,which may be one of the carcinogenesis modes in typeⅡ EC.