鸦胆子油乳注射液对胶质瘤细胞侵袭性的影响及其可能机制

Effect of Brucea Javanica Oil Emulsion on the Invasiveness of Glioma Cells and Its Possible Mechanism

目的探讨鸦胆子油乳注射液降低胶质瘤细胞侵袭性的作用与hosphatidylinositol 3kinase/protein kinase B(PI3K/AKT)信号通路之间的关系。方法采用鸦胆子油乳注射液处理培养的C6胶质瘤细胞,MTT法检测胶质瘤细胞的抑制率,Western blot法检测胶质瘤细胞内PI3K、AKT及核因子(NF)-κB蛋白的表达水平,结晶紫染色法观察透过Transwell下室面的胶质瘤细胞数。结果鸦胆子油乳注射液可以抑制PI3K、AKT及NF-κB的蛋白表达水平,且分别于鸦胆子油乳注射液作用30min、60min和120min时抑制程度最高。而鸦胆子油乳注射液作用180min时对C6胶质瘤细胞增殖的抑制作用最强,且此时Transwell下室面的胶质瘤细胞数最少。结论鸦胆子油乳注射液可能是通过抑制PI3K/AKT信号传导通路抑制胶质瘤细胞的增殖,进而降低胶质瘤细胞的侵袭性。

Objective To investigate the effect of brucea javanica oil emulsion on the invasiveness of glioma cells and hosphatidylinositol 3kinase/protein kinase B（PI3K/AKT）signaling pathway.Methods C6 glioma cells were treated by brucea javanica oil emulsion.The inhibition rate of glioma cells was detected by MTT,Western blot was used to detect protein expression levels of PI3 K,AKT,and nuclear factor（NF）-κB in glioma cells.The number of the glioma cells migrated through polycarbonate membrane was detected by crystal violet staining method.Results Brucea javanica oil emulsion inhibited PI3 K,AKT,and NF-κB protein expression which reached the highest inhibition at 30 min,60 min,and 120 min after brucea javanica oil emulsion,respectively.Maximum suppression on the proliferation of C6 glioma cells reached at 180 min after brucea javanica oil emulsion,while the number of glioma cells migrated through polycarbonate membrane was the least.Conclusion Brucea javanica oil emulsion inhibit the proliferation and invasiveness of glioma cells,which may be related to the inhibition of PI3K/AKT signal pathway.