调肝蠲浊颗粒对脂肪肝大鼠模型的调脂保肝功效及机制

Studies on particles of Tiao Gan Juan Zhuo for the model of nonalcoholic fatty liver in rats

目的研究调肝蠲浊颗粒对非酒精性脂肪肝大鼠模型的调脂保肝功效及机制。方法SpragueDawley(SD)大鼠10只(正常对照组)给予正常饲料喂养,50只大鼠给予高脂饲料喂养6w,造模成功后,随机分成5组:模型对照组、阳性对照组及调肝蠲浊颗粒低、中、高剂量组。除正常对照组和模型对照组灌胃生理盐水外,阳性对照组灌胃血脂康胶囊(0.12g·kg^(-1)·d^(-1)),调肝蠲浊颗粒低、中、高剂量组分别灌胃调肝蠲浊颗粒的水醇提取物,药物干预剂量依次为2.66、5.3、10.6g·kg^(-1)·d^(-1)。分别检测给药后大鼠血清中胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平,通过酶联免疫法(ELISA)检测大鼠血清中过氧化脂质(LPO)、C反应蛋白(CRP)的水平。结果血清血脂指标检测结果显示,与正常对照组比较,模型对照组TC、TG及LDL水平均显著升高,HDL水平有所下降,且差异有统计学意义(P<0.05);与模型对照组比较,调肝蠲浊颗粒3个剂量组TG水平无明显变化,TC、HDL以及LDL水平差异有统计学意义(P<0.05);与阳性对照组比较,调肝蠲浊颗粒高剂量组TC、HDL及LDL水平均降低,差异有统计学意义(P<0.05),其中调肝蠲浊颗粒高剂量组TC水平降低更明显。血清肝功指标检测结果显示,与正常对照组比较,模型对照组ALT和AST水平均明显升高,差异有统计学意义(P<0.05);与模型对照组比较,调肝蠲浊颗粒低、中、高3个剂量组ALT和AST水平的影响,依次呈现降低趋势,其中以高剂量组最为明显(P<0.05);高剂量组与低剂量组、中剂量组ALT和AST水平差异有统计学意义(P<0.05)。血清学指标检测结果显示,与正常对照组比较,模型对照组LPO与CRP水平均显著升高,差异有统计学意义(P<0.05);与模型对照组比较,阳性对照组以及3个剂量调肝蠲浊颗粒药物组LPO与CRP水平均大幅度下降,并以调肝蠲浊颗粒高剂量组最为明显,差异有统计学意义(P<0.05);与阳性对照组比较,调肝蠲浊颗粒高剂量组的CRP水平差异无统计学意义(P>0.05),其余剂量组差异均有统计学意义(P<0.05);高剂量组与低剂量组、中剂量组血清中LPO和CRP水平差异有统计学意义(P<0.05)。结论调肝蠲浊颗粒对高脂血症的治疗及肝脏的保护均具有一定疗效,以降低血清胆固醇水平最为显著,并且能够降低血清中LPO及CRP水平。

Objective To observe the influences of the regulation for liver on particles of Tiao Gan Juan Zhuo for the model of Hyperlipoidemia in rats.Methods Rats were given high fat diet for 6weeks to construct model of hyperlipidemia,then the particles of Tiao Gan Juan Zhuo was given.During the drug administration,the level of ALT,AST,TC,TG,HDL,LDL,LPO,CRP were measured.Results Compared with normal control group,TC,TG,and LDL levels of model control group increased significantly,HDL levels reduced significantly,the difference was statistically significant（P〈0.05）;Compared with model control group,the dosage of drug intervention on TG levels had changed,the TC,HDL and LDL levels all had different degrees of improvement,with statistically significant（P〈0.05）;Compared with positive control group,TC,HDL and LDL levels were statistically significant in high dose group（P〈0.05）;TC level was only statistically significant compared between dosage group（P〈0.05）.ALT and AST levels were significantly elevated in model control group compared with normal control group,with statistically significant（P〈0.05）;Compared with model control group,medicine group was with lower levels of ALT and AST,with statistically significant（P〈0.05）.Comparison between dosage group,give the dose and liver meritorious service is negatively related to the level of biochemical indicators of ALT and AST level,with statistically significant（P〈0.05）;Compared with positive control group,the differences between groups were no statistical significance（P〈0.05）.Compared with the positive control group,CRP level between cloud particles Tiaogan Juan high-dose group was not statistically significant（P〈0.05）,the remaining dose group differences were statistically significant（P〈0.05）;LPO and CRP serum levels in high dose group and low dose group was statistically significant（P〈0.05）.Conclusion Theparticles of Tiao Gan Juan Zhuo shows clear improvement for hyperlipidemia and the protection of liver.Reducing the serum is the most significant effect.The levels of serum LPO and CRP can reduced by Particles of Tiao Gan Juan Zhuo.