HPLC法测定紫锥菊不同药用部位中菊苣酸的含量

Determination of chicoric acid from different medicinal part of E.purpurea Moench by HPLC

目的研究紫锥菊不同药用部位中主要有效成分菊苣酸的含量情况,为紫锥菊药材的入药部位提供参考。方法：采用高效液相色谱法,流动相为乙腈-0.1%磷酸水（21：79）,流速为1 m L/min;检测波长：330nm。结果该法的平均回收率为103.26%,RSD为0.09%（n=9）。结论：菊苣酸在紫锥菊药材各个用药部位中均有分布,8~10月份采摘的全草含量最高。

Objective Research the content of chicoric acid from different medicinal part of E.purpurea Moench.The study provide the reference to medicinal part of E.purpurea Moench. Method： By the use of HPLC, mobile phase ： acetonitrile-0.1%Phosphoric acid water（21：79）, flow rate 1 m L/min, detection wavelength 330 nm. Result： The mean recovery of cichoric acid was 103.26%, RSD=0.09%（n=9）.Conclusion Each medicinal part of E.purpurea Moench is constituted with chicoric acid. The content of chicoric acid was most which from entire plants picked during August and October.