摘要
[ Objective] This study aimed to explore the presence of type I pill (fimC gene) and P pill (papC gene) and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill (fimC gene) and P pill (papC gene) were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill (fimC gene) ; 39.1% (9/23) of chick- en-derived E. coli strains expressed P pill (papC gene). In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% (6/14) of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.
[ Objective] This study aimed to explore the presence of type I pill (fimC gene) and P pill (papC gene) and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill (fimC gene) and P pill (papC gene) were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill (fimC gene) ; 39.1% (9/23) of chick- en-derived E. coli strains expressed P pill (papC gene). In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% (6/14) of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.
基金
Supported by National Natural Science Foundation of China(31072136)
Agricultural Science and Technology Achievement Transformation Project of the Ministry of Science and Technology of China(2012GB2A200044)
Project of Shijiazhuang Municipal Science and Technology Bureau(11150093A)