蒙脱石对百草枯中毒大鼠肝损伤的干预作用

The intervention of montmorillonite solution on liver injury induced by paraquat poisoning in rats

目的观察一次性灌胃百草枯染毒后大鼠的肝损伤及蒙脱石散甘露醇溶液的干预作用。方法 40只雄性SD大鼠按体重随机分为4组:对照组(C),百草枯染毒组(P),百草枯染毒1 h后进行蒙脱石散甘露醇溶液灌胃治疗组(PT1),百草枯染毒4 h后进行蒙脱石散甘露醇溶液灌胃治疗组(PT2)。结果大鼠百草枯染毒后一般状况差,血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、肝组织中超氧化物歧化酶(SOD)各组差异显著(P<0.05),丙二醛(MDA)无明显差异(P>0.05)。各染毒组较对照组ALT和AST含量明显升高(P<0.05),而肝组织中SOD含量则明显降低(P<0.05)。染毒后不同时间蒙脱石散甘露醇治疗组大鼠ALT和AST含量不同程度低于染毒组,仅PT1组与P组ALT差异显著(P<0.05),SOD高于染毒组(P>0.05)。各组大鼠肝组织切片无明显变化。结论百草枯一次性染毒可导致大鼠肝组织损伤,蒙脱石甘露醇溶液对百草枯中毒大鼠肝损伤的保护作用不明显。

Objective To observe the liver injury in rats by paraquat with intragastric administration once,and the effect of montmorillonite powder and mannitol on liver injury. Methods 40 male SD rats were randomly divided into four groups,control group（ C）,Paraquat group（ P）,PT1 groups treated with montmorillonite powder and mannitol gavage after paraquat exposure one hour,PT2 groups treated after paraquat exposure four hour. Results The general condition were poor in P group,PT1 group and PT2 group rats. The ALT and AST content of serum,the SOD of liver tissue in the rats showed significantly difference（ P〈0. 05）,while MDA level was not different（ P〈0. 05）. Compare with the control group,the ALT and AST content of P group increased significantly（ P〈0. 05）,while the SOD activity significantly decreased（ P〈0. 05）. ALT and AST in PT1 and PT2 group were lower than those in P group,while SOD activity was higher than P group＇s（ P〈0. 05）. Liver tissue of 4 groups had no significant diffenence,which meant paraquat didn＇t cause organic damage. Conclusion Paraquat exposure once time would lead to liver injury in rats. Protective effect of Montmorillonite powder and mannitol on liver injury induced by paraquat was not obvious.