基于铬天青S-铜-邻二氮菲传感体系对焦磷酸根的识别研究

Studies on chromazurol S-copper-phenanthroline complex toward recognition of pyrophosphate

铜离子与邻二氮菲、铬天青S能形成一种简单的化学传感体系,该体系在生理条件下对水溶液中焦磷酸根(PPi)的识别具有很好的选择性,PPi的加入会引起溶液颜色的变化(由蓝到黄),其他阴离子(F^-,Cl^-,Br^-,I^-,HSO_4^-,SO_4^(2-),Ac O^-,CO_3^(2-),C_2O_4^(2-),H_2PO_4^-,HPO_4^(2-),PO_4^(3-))的过量存在对PPi的识别基本无干扰。该体系在波长613 nm处的吸光度变化与PPi浓度在0~40μmol/L区间呈现良好的线性关系(r=0.994 3),检出限为1.69μmol/L,表明该体系可用于检测微量PPi。

A colorimetric chemosensing ensemble was prepared by simply mixing copper ion,phenanthroline with chromazurol S in water solution. The ensemble showed highly selective recognition to pyrophosphate（ PPi） under physiological condition. Addition of PPi induced a noticeable color change from blue to yellow. Other anions such as F^-,Cl^-,Br^-,I^-,HSO_4^-,SO_4^（2-）,Ac O^-,CO_3^（2-）,C_2O_4^（2-）,H_2PO_4^-,HPO_4^（2-）,PO_4^（3-）do not produce significant influence on PPi recognition process. The absorbance change at613 nm exhibited a good linear relationship with PPi concentration in the range of 0 ~ 40 μmol/L（ r =0. 994 3）. The detection limit of the ensemble to PPi was 1. 69 μmol/L,and suggesting that could be used in the detection of micromolar PPi.