EdCC保护乳小鼠心肌细胞氧化应激所致细胞损伤及凋亡

EdCC Protective Effect on Neonatal Cardiomyocytes from Oxidative Stress Induced Injury and Apoptosis

目的：观察子宫内膜干细胞（En SCs）来源细胞因子＂鸡尾酒＂（EdCC）对乳小鼠心肌细胞氧化应激损伤和凋亡的保护作用。方法：乳小鼠心肌细胞分为正常组、对照组和EdCC组,用过氧化氢（H2O2）造成氧化应激,检测上清液乳酸脱氢酶（LDH）活性,TUNEL染色检测细胞凋亡,DCFH-DA检测细胞内活性氧族（ROS）浓度。结果：En SCs呈成纤维细胞样贴壁生长,EdCC蛋白提取效率为每24 h提取（241.3±32.1）μg/10~6个细胞;与对照组比较,EdCC组（100 mg/L）上清液LDH活性明显降低,心肌细胞凋亡率明显降低,细胞内ROS浓度降低,差异均有统计学意义（P〈0.01）。结论：EdCC减轻体外培养乳小鼠心肌细胞氧化损伤及凋亡,可能与降低细胞内ROS水平有关。

Objective： To investigate the protective effect of endometrial stem cells（ En SCs） derived from cytokine cocktail（ EdCC） on neonatal mouse cardiomyocytes under oxidative stress induced injury and apoptosis. Methods： Neonatal mouse cardiomyocytes were divided into normal group,control group and EdCC group. H2O2 was used to induce oxidative stress. The lactate dehydrogenase（ LDH）activity in supernatants was determined to evaluate cell damage,TUNEL staining was used to count apoptotic cells and DCFH-DA was used to measure intracellular reactive oxygen species（ ROS） concentration. Results： En SCs were adherent fibroblast-like cells and the efficiency of EdCC concentration was（ 241. 3 ± 32. 1） g /10~6 cells in every 24 h. As compared with control group,the LDH activity in supernatants greatly reduced in EdCC group（ EdCC 100 g / m L）（ P〈0. 01）,and also the apoptotic cardiomyocyte ratio（ P〈0. 01） and intracellular ROS concentration（ P〈0. 01）,difference was statistical significant. Conclusion： EdCC alleviated oxidative stress induced injury and cell apoptosis in neonatal mouse cardiomyocytes,possibly associated with decreasing intracellular ROS level.