灵芝三萜类化合物对AD大鼠海马神经元凋亡的影响

Effects of Ganoderma Lucidum Triterpenoids on Apoptosis of Brain Tissue of Alzheimer Disease Model Rat

目的:研究灵芝三萜类化合物(GLT)对阿尔茨海默病(AD)模型大鼠脑细胞凋亡的影响。方法:将自然衰老模型大鼠60只随机均分为GLT低、中、高剂量组,模型组,溶媒对照组(食用油)及阳性对照组(健脑胶囊),5月龄大鼠10只为正常对照组;连续灌胃给予相应药物60 d,取各组大鼠海马组织HE染色观察神经元变化,TUNEL法检测各组大鼠海马神经细胞凋亡。结果:与正常对照组比较,模型动物脑海马神经细胞损伤明显,细胞排列极度紊乱,大小形态不规则,数量明显减少,海马凋亡阳性细胞数显著增多,差异具有统计学意义(P<0.05),神经细胞核内有大量深染棕黄色颗粒状;给予GLT后,神经细胞损伤减轻,GLT高剂量组无明显神经细胞坏死特征;与模型组比较,GLT中、高剂量组大鼠海马凋亡阳性细胞数减少(P<0.05),神经细胞核浅染棕黄色颗粒。结论:GLT可减少AD大鼠海马神经元凋亡,这可能是防治AD的机制之一。

Objective： To investigate the protective effects and mechanism of Ganoderma lucidum Triterpenoids（ GLT） in Alzheimer Disease model rats. Methods： 60 natural aging rats were randomly divided into 6 groups,GLT low dose group,GLT middle dose group,GLT high dose group,model group,solvent group（ edible oil） and positive group（ JNJN）. 10 rats of 5 months of age were used as control group. Then the intragastric administration was used. After being treated with GLT for 60 days,brain tissues were stained with Hematoxylin-eosin to observed,and the numbers of apoptotic neuron in hippocampus were tested by TUNEL. Results： Neurons were damaged in the brain,cell display were extremely messed with irregular size and shapes and the number of cells decreased in model rats. Hippocampus apoptotic positive cells increased significantly,large amount of dark brown particles were found in neuro cell nucleus; GLT high dose group showed no significant neuro cell apoptosis features;comparing with model group,middle and high dose group showed decreased hippocampus apoptotic positive cells（ P〈0. 05）,light brown particles were found in neuro cell nucleus. Conclusion： GLT can decrease necrosis of neuron and inhibit apoptosis,it maybe the mechanisms of protecting AD model animals.