斜线电泳分离线粒体白蛋白前体蛋白（英文）

Identification of albumin precursor protein from the mitochodria by the diagonal gel electrophoresis

目的:分离并鉴定线粒体内的白蛋白前体蛋白。方法:采用离心法分离大鼠肝及肾线粒体,采用斜线电泳法(第一相非还原SDS-PAGE,第二相还原SDS-PAGE)分离线粒体白蛋白前体蛋白,采用质谱肽测序法鉴定蛋白质。结果:斜线电泳胶上斜线上方出现一个点,该点经质谱肽测序进一步鉴定为白蛋白前体蛋白。结论:斜线电泳联合质谱肽测序能鉴定出线粒体中的白蛋白前体蛋白。

Objective： to separate and indentify the albumin precursor protein from the mitochodria. Methods： the mitochodria were separated from rat liver or kidney by centrifugation; the albumin precursor protein was separated from the mitochodria by the diagonal gel electrophoresis with the first non-reducing SDS-PAGE and the second reducing SDSPAGE. Protein was identified further by mass spectrometric peptide sequencing. Results： there was a distinct spot that ran above the diagonal line in the gel after the diagonal gel electrophoresis; the albumin precursor proteins were identified in this spot by the mass spectrometric peptide sequencing. Conclusion： the albumin precursor protein from the mitochodria can be identified by the diagonal gel electrophoresis method with the mass spectrometric peptide sequencing.