青蒿素在小鼠花粉过敏性结膜炎早期反应相中的作用

Effects of artemisinin on early-phase response of allergic conjunctivitis in a murine model induced by pollen

目的：探讨青蒿素对小鼠花粉过敏性结膜炎早期反应相的免疫抑制作用。方法：选取Balb/c雌性小鼠25只,随机分为五组：A组：模型组;B组：青蒿素滴眼组;C组：青蒿素灌胃组;D组：地塞米松磷酸钠滴眼组;E组：阴性对照组。初次诱导采用小鼠尾根部及足垫皮下注射豚草花粉粗浸液与弗氏佐剂的混合液,第7d和14d加强致敏采用腹腔注射。阴性对照组使用等量的弗氏佐剂与PBS混合液代替。在第21~27d进行治疗,青蒿素滴眼组给予1%青蒿素滴眼液滴眼,4次/d;青蒿素灌胃组按600mg/kg的剂量进行灌胃,1次/d;地塞米松磷酸钠滴眼组给予地塞米松磷酸钠滴眼液滴眼,4次/d;模型组及阴性对照组给予青蒿素滴眼液基质液滴眼。治疗后采用豚草花粉变应原滴眼激发后1h内,取眼球用于组织病理学检测,取血清用于豚草花粉特异性IgE测定。结果：模型组小鼠激发后过敏症状较其他各用药组明显严重;地塞米松磷酸钠滴眼组、青蒿素滴眼组及灌胃组过敏症状评分、肥大细胞脱颗粒比率及血清中豚草花粉特异性IgE浓度均高于阴性对照组,而显著低于模型组。结论：青蒿素滴眼及灌胃治疗均可明显抑制小鼠花粉过敏性结膜炎症状、早期反应相中肥大细胞脱颗粒比率和豚草花粉特异性IgE的产生,推测青蒿素对花粉过敏性结膜炎具有一定的治疗作用。

AIM ： To investigate the immunosuppression of artemisinin in the early-phase response（ EPR） of allergic conjunctivitis in a murine model induced by pollen.METHODS： Twenty-five Balb /c mice were divided into five groups at random： A： model group,B： artemisinin topical treatment group,C： oral artemisinin group,D：dexamethasone topical treatment group, E： negative control group. Balb / c mice were first sensitized with mixture of ragweed crude extract and complete Freund＇s adjuvant by left footpad and root of tails injection at days0. At days 7 and 14,mice were strengthen sensitized by intraperitoneal injection. Negative control group replacd with a mixture of equal amounts of Freund＇s adjuvant and PBS mixture. Treatment time was between 21-27d：group B received 1% topical artemisinin eye drops 4times per day; group C received artemisinin at doses of600 mg / kg orally once per day; group D received topical dexamethasone eye drops 4 times per day. The model and naive groups replaced with solvent for control. After treatment,mice were excited by ragweed pollen crude infusion drops. Within 1h after the excitation,the eyes were taken for histopathology testing, the serumfor detection of specific IgE for ragweed pollen.RESULTS： After excitation,allergy symptoms of model group were more significantly compared with negative control group and the treatment groups; allergy symptomscore,mast cell degranulation ratios and the ragweed pollen specific IgE in serumin the topical treatment groups and oral group were higher than that in the negative control group,but significantly lower than that in model group.CONCLUSION： Artemisinin topical treatment and oral treatment for mice could inhibit the symptoms of pollen allergic conjunctivitis,early-phase response of mast cell degranulation ratio and specific IgE generation for ragweed pollen,suggesting that artemisinin has some therapeutic effect for pollen allergic conjunctivitis.