固相萃取-高效液相色谱法测定天然维生素E中苯并(a)芘

Determination of benzo( a) pyrene in natural vitamin E by solid phase extraction-high performance liquid chromatography

建立了固相萃取-高效液相色谱测定天然维生素E中苯并（a）芘的方法。天然维生素E样品经Cleanert Silica串联Cleanert Bap固相萃取柱前处理净化后采用C18色谱柱分离,以乙腈-水（体积比80∶20）为流动相,荧光检测器检测。结果表明：苯并（a）芘的质量浓度在0.5~10μg/L范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 8,方法检出限为0.3μg/kg;样品加标回收率在88%~96%之间,相对标准偏差在2.8%~3.6%之间。该方法简单便捷,灵敏度高,选择性好,适用于天然维生素E中苯并（a）芘的测定。

The method for determination of benzo（a） pyrene in natural vitamin E was established by solid phase extraction - high performance liquid chromatography. After the natural vitamin E samples were purified by Cleanert Silica tandem solid phase extraction column Cleanert Bap pretreatment, they were separated on C18 chromatographic column using acetionitrile and water （volume ratio 80： 20） as mobile phase, and detected by FLD. The results showed that the mass concentration of benzo（a） pyrene had good linear relationship with chromatographic peak area in the range of 0.5 - 10μg/L , and the correlation coefficient and limit of detection were 0. 999 8 and 0.3μg/kg, respectively. The recovery rate of standard addition was 88% - 96%, simpleness, high pyrene in natural and the relative standard deviation was 2.8% - 3.6%. The method had the items of sensitivity and good selectivity, which was suitable for the determination of benzo （a） vitamin E.