参麦注射液对高脂血症大鼠血脂的调节作用

Modulating effects of shenmai injection on serum lipids in rats with hyperlipemia

目的:通过观察参麦注射液对高脂血症模型大鼠的血脂水平和一系列相关生化指标的影响,探讨其对高脂血症模型大鼠血脂的调节作用。方法:30只SPF级雄性SD大鼠用基础饲料适应性喂养1周,随机分为3组(n=10):对照组,模型对照组,参麦注射液组。对照组用基础饲料喂养,模型对照组和参麦注射液组用高脂饲料喂养,每周测定动物体重一次。参麦注射液组每天给予2次参麦注射液10 ml/kg,连续灌胃45 d。之后测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)、丙二醛(MDA)、脂蛋白酯酶(LPL)和肝酯酶(HL)活性。结果:模型对照组大鼠体重、血清中TC、TG、LDL-C和MDA水平较对照组明显升高(P<0.01),而HDL-C、SOD、GSH-Px、LPL和HL水平明显降低(P<0.01)。参麦注射液组大鼠体重、血清中TC、TG、LDL-C和MDA水平较模型对照组明显降低(P<0.01),而HDLC、SOD、GSH-Px、LPL和HL水平明显升高(P<0.05,P<0.01)。结论:参麦注射液对高脂模型大鼠的血脂具有较明显的调节作用,并具有抗脂质过氧化的作用。

Objective： To discuss the modulating effects of shenmai（Traditional Chinese Medicine） injection on blood lipid in hyperlipidemia rats through observing the serum lipid level and a series of related biochemical indexes in hyperlipidemia rat model. Methods： A total of 30 male SD rats of SPF grade were purchased and fed with basic feed for 1 week to adapt the environment. Then the rats were randomly divided into the following groups（n =10）： control group,model control group,shenmai injection group. Control group was fed basal diet; the latter two groups were fed high fat diet,the body weight of all the animals was measured each week. For shenmai injection group,the rats were fed with shenmai injection（10 ml / kg） twice a day for 45 consecutive days through oral administration. The effects of shenmai injection on body weight,serum triglyceride（TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C）,super oxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,malondialdehyde（MDA）,the activities of lipoprotein lipase（LPL） and hepatic lipase（HL） in hyperlipidemia rats were detected. Results： Body weight,serum of TC,TG,LDL-C,HDL-C,and MDA levels in model control group were significantly higher（ P 0. 01）,while serum of HDL-C,SOD,GSH-Px,LPL,and HL levels were significantly lower than those of control group（ P 0. 01）. Body weight,serum of TC,TG,LDL-C,HDL-C,and MDA levels in shenmai injection group were significantly lower（ P 0. 01）,while serum of HDL-C,SOD,GSH-Px,LPL,and HL levels were significantly higher than those of model group（ P 0. 05,P 0. 01）. Conclusion： Shenmai injection has a significant effect of modulating blood lipid and antioxidant function on hyperlipidemia rats.