CD44和CD24标记的宫颈癌细胞亚群特性

Characteristics of CD44 and CD24-marked Cervical Cancer Cells Subgroups

目的考察Siha细胞系中的CD44^+/CD24^+能否富集宫颈癌干细胞。方法用流式细胞仪分选出CD44^+/CD24^+Siha细胞,用无血清悬浮培养观察成球能力、裸鼠移植瘤实验观察成瘤能力、透射电子显微镜观察辐射前后两组细胞形态变化,并通过Transwell侵袭实验比较细胞侵袭能力的差异。结果耐放疗细胞中CD44^+/CD24^+Siha细胞比例明显高于其在亲代Siha细胞中的比例;无血清培养CD44^+/CD24^+Siha细胞组可以形成致密且体积较大的细胞球,CD44^+/CD24^+Siha细胞组致瘤时间早,成瘤率高;辐射后CD44^+/CD24^+Siha细胞较亲代Siha细胞更抗凋亡;CD44^+/CD24^+Siha细胞组的迁移细胞数明显高于亲代Siha细胞组,所有数据均具有统计学意义。结论 CD44^+/CD24^+Siha具备部分干细胞特性,CD44^+/CD24^+可能成为宫颈癌干细胞特异性表面标志物。

Objective To investigate the enrichment feasibility of cancer stem cells（CSC） in CD44~＋/CD24~＋Siha cells. Methods CD44~＋/CD24~＋Siha cells were acquired by flow cytometry. The capacities of cells spherical clusters of CD44~＋/CD24~＋Siha cells and parent Siha cells were observed. Tumor xenografts tests were used to evaluate tumor-forming ability. The changes of cells morphology of the two groups after irradiation were observed by transmission electron microscopy. Migration and invasiveness were assessed by Transwell tests. Results The proportion of CD44~＋/CD24~＋ cells in radioresistant cells was higher than that in parent Siha cells. Compared with parent cells, CD44~＋/CD24~＋Siha cells cultured in serum free environment can form more compacted and larger-volume cell clusters. Not only the time to form tumors is shorter but also the efficiency is higher in the CD44~＋/CD24~＋Siha cells than parent cells. The irradiated CD44~＋/CD24~＋Siha cells show the higher ability to resist apoptosis than their parent cells. The migration cell numbers in CD44~＋/CD24~＋cells are much more than those in parent cells. All data are statistically significant. Conclusion The CD44~＋/CD24~＋Siha cells exhibit the characteristics of stem cells and maybe as a surface marker for the identification of stem cells from cervical cancer cells.