粗根荨麻多糖对人脐血单核细胞源树突细胞表面分子表达的影响

Urtica macrorrhiza Hand-Mazz Polysaccharides Induce Cord Blood Monocytes into Mature Dendritic Cells( DCs)and Its Effect on Surface Molecules Expression of DCs

研究粗根荨麻多糖在体外对人脐血单核细胞向树突细胞分化及成熟的作用。用淋巴细胞分离液分离脐血获得脐血单个核细胞,将获得的单核细胞分为3组,实验组:在含有粗根荨麻多糖(200 mg/L)的RPMI1640完全培养液中培养;阴性对照组:在无药物的RPMI1640完全培养液中培养;阳性对照组:在含有细胞因子(IL-4、GM-CSF、TNF-α)的RPMI1640完全培养液中培养;收集第10 d细胞用流式细胞术检测DCs成熟表型(CD1a、CD80、CD83、CD86、HLA-DR)。结果表明,与阴性对照组比较,实验组和阳性对照组CD80、CD83、CD86、HLADR、CD1a的表达明显增加。粗根荨麻多糖可诱导脐血单核细胞分化成熟的DCs。

To study the effect of Urtica macrorrhiza Hand-Mazz polysaccharides on in vitro induction of cord blood monocyte into dendritic cells（DCs） as well as its effect on the maturation and differentiation of DCs. Cord blood mononuclear cytes were separated by centrifuge in density gradient and were divided into three groups：cells cultured with U. macrorrhiza polysaccharides （200 mg/L） as experimental group, cells cultured with the cytokines of IL-4, GM-CSF and TNF-α as positive control group and cells cultured without either IL-4, GM-CSF, TNF-α or U. macrorrhiza polysaccharides as negative control group. The phenotype of 10 days cultures of DCs （CD1a, CDS0, CD83, CD86 and HLA-DR） were identified by flow cytometry. The results showed that compared with negative control group, cells of experimental group and positive group cultured for 10 days expressed significant increasing of the phenotypes of CD80, CD83, CD86, HLA-DR and CD1 a by flow cytometry. Hence, it was concluded that U. macrorrhiza polysaccharides, may induce the cord blood monocytes into mature DCs.