62株多重耐药鲍曼不动杆菌耐药性分析及同源性研究

Investigation on drug resistance and homology analysis of 62 strains of multidrug-resistant Acinetobacter baumannii

目的对多重耐药鲍曼不动杆菌(MDRAB)进行耐药性分析及同源性研究,为预防与控制院内感染提供理论依据。方法收集、分离及鉴定多重鲍曼不动杆菌62株,采用琼脂稀释法检测菌株对23种抗菌药物的最低抑菌浓度(MIC),脉冲场凝胶电泳(PFGE)进行分子分型并分析其同源性。结果 62株MDRAB中,对大部分抗菌药物的耐药率在80.0%以上,PFGE将其分为4型,A型和B型为主要克隆株。A型包括4个亚型共32株,B型有2个亚型共19株,C型有8株,D型有3株。结论 MDRAB耐药情况十分严峻,在临床科室广泛分布并流行传播,成为耐药菌株逐年增加的一个重要因素。

Objective To analyze the drug resistance and homology of multi-drug resistant Acinetobacter baumannii（MDRAB）to provide a theoretical basis for the prevention and control of nosocomial infection.Methods A total of 62 strains of MDRAB were collected,separated and identified.The minimum inhibitory concentration（MIC）of 23 kinds of antimicrobial drugs in these strains were detected by the agar dilution method.Molecular typing and homology analysis for these strains was analyzed by using the pulsed field gel electrophoresis（PFGE）.Results Among 62 strains of MDRAB,the resistance rates to most of antibacterial drugs were above 80.0%.The four types were classified by PFGE,the type A and B were the main cloned strains.The type A included 4subtypes and 32 strains,the type B had 2subtypes and 19 strains,and the type C had 8 strains and type D had 3 strains.Conclusion The drug resistance situation of MDRAB is very serious,MDRAB is widely distributed,popularized and communicated in clinical departments,which becomes an important factor for drug resistant strain increase year by year.