γ干扰素诱导蛋白-10在强直性脊柱炎患者血清中的表达及其临床意义

Expression of interferon-γ-inducible protein-10 in serum of patients with ankylosing spondylitis and its clinical significance

目的检测强直性脊柱炎(AS)患者血清中γ干扰素诱导蛋白-10(IP-10)的表达水平,分析IP-10与疾病的关系,评价其在AS疾病活动中的临床意义。方法选取AS活动期患者34例、AS稳定期患者24例及体检健康者46例作为研究对象,采用ELISA法检测血清中IP-10、IL-17、IL-23和TNF-α水平,分析血清IP-10水平与AS各临床和实验室指标的相关性。结果 AS活动期组及稳定期组患者血清IP-10含量水平均显著高于健康对照组(P<0.05);IP-10在AS活动期组含量水平高于稳定期组(P<0.05);两组AS患者血清IL-17、IL-23及TNF-α含量水平均高于健康对照组(P<0.05);IL-17和TNF-α在AS活动期组含量水平高于稳定期组(P<0.05)。相关性分析表明,AS患者中IP-10与IL-17、IL-23、TNF-α、PLT、ESR、CRP和BASDAI评分呈正相关。结论血清中IP-10水平可能是AS发病过程中重要的免疫学标志,检测IP-10有助于对AS的诊断和病情的评估。

Objective To investigate the levels of serum interferon-γ-inducible protein 10（ IP-10） in patients with ankylosing spondylitis（ AS）,and evaluate its clinical significance in the disease activity of AS. Methods The levels of IP-10,IL-17,IL-23 and TNF-αin serum from 34 patients with active ankylosing spondylitis,24 patients at stable phase and 46 healthy controls were detected by enzyme linked immunosorbent assay（ ELISA）. The correlation of serum IP-10 level with AS-associated clinical and laboratorial indexes were analyzed. Results The levels of serum IP-10 in active and stable phase AS patients were significantly higher than that of healthy controls（ P〈0. 05）,and the serum IP-10 level in active AS patients was significantly higher than that in inactive AS patients（ P〈0. 05）. The levels of serum IL-17,IL-23 and TNF-α in AS patients were higher than that of healthy controls（ P〈0. 05）,and the levels of serum IL-17 and TNF-α in active AS patients were significantly higher than those of inactive AS patients（ P〈0. 05）. Correlation analysis showed that the level of IP-10 was positively correlated with IL-17,IL-23,TNF-α,PLT,ESR,CRP,BASDAI score in AS patients. Conclusion The serum IP-10 level may be an important immune marker in the process of AS,and it can be helpful to diagnose and evaluate AS.