二甲苯、蒽、苯并[α]芘对虾夷扇贝幼贝血清SOD、CAT活性的影响

Effects of Xylene,Anthracene,and Benzo[α]pyrene on Enzymatic Activities of SOD and CAT in the Japanese Scallop( Mizuhopecten yessoensis) Serums

以虾夷扇贝(Mizuhopecten yessoensis)为受试生物,设置3种不同浓度的二甲苯、蒽、苯并[α]芘处理健康的虾夷扇贝,检测血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性变化情况,结果发现:0.01~1.0 mg·L^(-1)的二甲苯、0.002~0.2 mg·L^(-1)的蒽、0.001~0.1 mg·L^(-1)的苯并[α]芘处理6、12 h后,虾夷扇贝血清SOD活性升高,处理12 h后对血清SOD活性诱导率均高于处理6 h的;处理6 h后,血清CAT活性降低,而处理12 h后,血清CAT活性升高;具有显著的剂量-效应关系和时效关系,表明二甲苯、蒽、苯并[α]芘对虾夷扇贝具有氧化胁迫作用,可能导致其氧化损伤。相关性分析发现,3种污染物处理6 h后,血清SOD活性与CAT活性变化呈显著负相关;而处理12 h后,血清SOD活性与CAT活性变化呈显著正相关。上述结果为开展二甲苯、蒽、苯并[α]芘对海洋贝类毒性评价提供基础数据。

The present study is aimed to evaluate the effects of xylene,anthracene and benzo[α]pyrene on the activities of superoxide dismutase（ SOD） and catalase（ CAT） in the Japanese scallop（ Mizuhopecten yessoensis） serums. Three different concentrations of xylene,anthracene and benzo[α]pyrene were selected to treat the healthy Japanese scallop individuals. The results showed that the SOD activities of Japanese scallop serums were remarkably induced at 6 and 12 hours after the treatments of 0. 01^-1. 0 mg·L^-1xylene,0. 002-0. 2 mg·L^-1 anthracene and 0.001-0. 1 mg·L^-1 benzo[α]pyrene. Compared to controls; the CAT activities were remarkably inhibited at 6 h,but induced at 12 h,showing remarkable dose-effect and time-effect relationship. This suggested that the xylene,anthracene and benzo[α]pyrene had oxidative stress on Japanese scallop,and might cause oxidative damages. Correlation analysis showed that the SOD activity was remarkably negative correlated with the CAT activity of Japanese scallop serums treated with three pollutants at 6 h,but SOD activity was remarkably positive correlated with the CAT activity at 12 h. These results will provide the basic data for toxic assessment of the xylene,anthracene and benzo[α]pyrene on the Japanese scallop.