苯并芘通过NF-κB诱导RAW264.7细胞MMPs的表达

Benzo[a]pyrene(BaP) Induced the Expression of MMPs in the RAW264.5 Cell through the NF-κB

目的探讨烟雾主要成分苯并芘(Ba P)对RAW264.7单核-吞噬细胞中基质金属蛋白酶(MMPs)的影响,为研究吸烟与腹主动脉瘤的关联提供相关依据。方法应用免疫印迹法观察Ba P对体外培养的RAW264.7单核-吞噬细胞基质金属蛋白酶-2/9/12(MMP-2/9/12)的影响,并验证其是否通过NF-κB信号通路。结果 Ba P暴露使培养巨噬细胞NF-κB活化,同时伴随MMP-9、MMP-12表达升高,而PDTC,核转录因子NF-κB的抑制剂,可抑制NF-κB活化,同时伴随MMP-9、MMP-12表达下降。结论 Ba P可通过NF-κB通路诱导RAW264.7单核-吞噬细胞MMP-9、MMP-12表达升高。

Objective To explore the effect of benzopyrene （BaP）, the maincomponents of smoke, on matrix metalloproteinase （MMPs） of RAW246.7 mononuclear macrophage. Methods We observed the influence of BaP on matrix metalloproteinase - 2/9/12 （ MMP - 2/9/12） of RAW246.7 mononuclear macrophage in vitro by western blotting and verified if it happened through the NF - κB signaling pathway. Results BaP caused NF - κB activation and expression of MMP - 12, MMP - 9 increased in cultured macrophages. While PDTC, an inhibitor of nuclear transcription factor NF - κB, caused the inhibition of NF - KB activation and expression of MMP - 12 and MMP - 9 decreased. Conclusion BaP can induce the expression of MMP - 9 and MMP - 12 in RAW246.7 cell by NF - κB pathway.