兰州地区健康成人静脉血网织红细胞相关参数参考值的建立

Establish the reticulocyte parameters of vein blood of healthy adults in Lanzhou area

目的通过对兰州地区1 880名健康成人网织红细胞（RET）及分群参数的调查研究,建立本地区RET及分群值的参考区间。方法采用回顾分析法,用Sysmex XE-5000全自动血细胞分析仪进行检测分析。结果各参数中除RET、高荧光强度RET比率（HFR）接近正态分布外,其余均为偏态分布;RET参考范围,男性为0.50%~1.80%,女性为0.45%~1.76%;低荧光强度RET比率（LFR）参考范围,男性为89.16%~99.70%,女性为91.20%~99.60%;中荧光强度RET比率（MFR）参考范围,男性为0.30%~8.31%,女性为0.20%~9.94%;HFR参考范围,男性为0.01%~0.90%,女性为0.01%~1.10%,未成熟RET比率（IRF）参考范围,男性为0.40%~8.80%,女性为0.30%~10.60%;RET血红蛋白含量（RET-HE）参考范围,男性为37.00~47.11pg,女性为35.45~45.35pg;除LFR和HFR外,男女间参考范围差异具有统计学意义（P〈0.05）;不同年龄组间除HFR（P=0.158）外,其他各参数组间差异均具有统计学意义（P〈0.05）。结论 RET分群参数较原参考值发生较大改变,原参考值缺乏准确性和适用性,有必要科学、合理地建立地区性的参考值范围。

Objective Through the investigation on the parameters of reticulocyte（RET）and grouping of 1 880 healthy adult in Lanzhou area,to establish the reference range of the RET and grouping parameters of Lanzhou area.Methods Using the retrospect analysis,the parameters was determined by Sysmex XE-5000 full automation analyser.Results In all parameters,only the RET and HFR were close to normal distribution,and the others appear were skew distribution.The reference range of RET% was 0.50%to1.80%in male,0.45%to 1.76%in female.The reference range of LFR was 89.16%to 99.70%in male,91.20% to 99.60%in female.The reference range of MFR was 0.30%to 8.31%in male,0.20%to 9.94%in female.The reference range of HFR was0.01%to 0.90% in male,0.01% to 1.10% in female.The reference range of IRF was 0.40% to 8.80% in male,0.30% to10.60%in female.The reference range of RET-HE was 37.00 to 47.11 pg in male,35.45 to 45.35 pg in female.Except for LFR and HFR,difference between male and female of the range of reference was statistically significant（P〈0.05）.Except for HFR（P=0.158）,in different age groups,the difference was statistically significant（P〈0.01）.Conclusion RET grouping parameters change greatly compared with the original reference values,the original reference value is lack of accuracy and applicability,and it is necessary to establish regional reference range scientificly and reasonably.