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罗非鱼胰岛素样生长因子Ⅰ(IGF-Ⅰ)的生物信息学分析

Bioinformatics analysis of insulin-like growth factor-Ⅰ(IGF-Ⅰ)in nile tilapia
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摘要 为进一步研究罗非鱼胰岛素样生长因子Ⅰ(IGF-Ⅰ)的生物学特征,根据NCBI上登录的尼罗罗非鱼的IGF-Ⅰ氨基酸序列,利用相关的软件对其进行生物信息学分析。结果显示,罗非鱼IGF-Ⅰ蛋白含有182个氨基酸,pI为9.65,脂溶指数为51.98,是不稳定的脂溶性亲水蛋白。二级结构中,60.99%为环,31.32%为螺旋。IGF-Ⅰ蛋白具有信号肽,含有4个二硫键,具有一个跨膜区。IGF-Ⅰ蛋白具有13个磷酸化、16个糖基化和12个泛素化等蛋白质翻译后修饰位点。 In order to study the biological characteristics of IGF-Ⅰin tilapia,bioinformatics were analyzed using biology softwares,according to amino acid sequence of tilapia IGF-Ⅰfrom NCBI.IGF-Ⅰof tilapia was composed of 182 amino acid residues.The theoretical Pi and aliphatic index was 9.65 and 51.98,respectively.IGF-Ⅰwas basic,fat-soluble and unstable protein.There were 60.99%loop and 31.32%in IGF-Ⅰ secondary structure.There were a signal peptide and four disulfide bonds in IGF-Ⅰ.It had one transmembrane.There were some post-translation modified sites in IGF-Ⅰ,including 16O-glycosylation site,13 phosphorylation sites and 12 ubiquitination sites.
作者 王伟伟
机构地区 山西农业大学动物科技学院
出处 《山西农业大学学报（自然科学版）》 CAS 2016年第6期422-427,共6页 Journal of Shanxi Agricultural University(Natural Science Edition)
基金 山西农业大学创新基金(2010025)
关键词 罗非鱼 IGF-Ⅰ 生物信息学 Nile tilapia IGF-Ⅰ Bioinformatics analysis
分类号 S937.3 [农业科学—渔业资源]
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山西农业大学学报（自然科学版）
2016年 第6期

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