人皮肤慢性光老化成纤维细胞环状RNA的差异表达

Differentially Expression of Circular RNAs in Human Fibroblast Cells Related to Skin Photoaging

目的:构建人皮肤慢性光老化成纤维细胞模型,筛查与光老化相关的环状RNA(circRNA)。方法:分离培养成纤维细胞,利用UVA多次照射,制作人皮肤慢性光老化成纤维细胞模型,采用β-半乳糖苷酶染色及Western印迹法检验p21蛋白验证,以给予与照光时长等同的避光处理的成纤维细胞作为对照。采用RNAseq高通量转录组测序技术检测光老化成纤维细胞中circRNA表达,并分析其差异表达的circRNA。结果:与对照组比较,光老化模型组成纤维细胞中表达显著上调的circRNAs共有7个(P<0.05);表达显著下调的circRNAs共有4个(P<0.05)。经生物信息学分析,差异表达的circRNA可能与胶原蛋白合成等基因有关。结论:光老化模型组成纤维细胞存在多个差异表达的circRNAs,说明circRNA可能参与光老化的基因调控。

Objective：To screen and identify the differentially expressed circRNA in photoaged model of human fibroblasts. Methods： Human dermal fibroblasts （HDFs） at early passages were exposed to a series of sub-cytotoxic dose of UVA. The senescent phenotypes were detected by the expression of ^-galaetosidase and p21 protein. Circular RNAs expression were identified in pho- toaged HDFs through high-throughput deep sequencing coupled with analysis of massive transcrip- tional data. Results：Sequencing result revealed that 11 eircRNAs were significantly altered in the photoaged model （P 〈 0. 05 ） compared with the control group. Among them, 7 were significantly up-regulated, and the other 4 were down-regulated. Bioinformaties analysis revealed that those cir- cRNAs may involve in encoding the chains of collagen. Conclusion：This study results confirmed some circRNAs were differentially expressed in photoaged HDFs model, which may participate in the process of gene regulation of photoaging.