摘要
为了解不同母源抗体水平对仔猪免疫后抗体消长的影响,以及免疫后细胞因子的变化,使用口蹄疫O型灭活疫苗对40头初生仔猪进行免疫,并于初次免疫后28 d二次免疫,采用液相阻断ELISA方法检测血清中抗体滴度,双抗体夹心ELISA法检测血清IL-8和IL-12水平。结果显示,母源抗体水平越高对于初次免疫后仔猪抗体影响越大;二次免疫后抗体水平可长时间维持较高水平。初次免疫后14 d^28 d,血清中IL-8和IL-12水平上升极显著(P<0.01);二次免疫后14~28 d,IL-8水平上升极显著(P<0.01),IL-12水平上升不显著(P>0.01)。试验表明,母源抗体水平是影响仔猪免疫后抗体消长的因素之一;口蹄疫O型灭活疫苗能有效提高血清中IL-8和IL-12水平。
In order to study the influence of different maternal antibody levels on the dynamics of antibody and the changes of cytokines after piglet immunization,type O FMD inactivate vaccine was used to immune 40newly- born piglets,and second immunization on day 28 post primary immunity. The antibody titer in serum was detected by liquid- phase blocking ELISA,the levels of IL- 8 and IL- 12 in serum were detected by double antibody sandwich ELISA. Results show that the higher maternal antibody level was,the greater effect of antibody on piglets after first immunization,the antibody level can maintain a high level for a long time after booster immunity. 14 to 28 days after first immunity,the levels of IL- 8 and IL- 12 in serum are greatly significant（ P〈0. 01）; 14 days to 28 days after second immunity,the level of IL- 8 is greatly significant（ P〈0. 01）; the increase of IL- 12 level is not significant（ P〉 0. 05）. Results show that maternal antibody level is one of influencing factors of the dynamics of antibody after piglet immunization; type O FMD inactivate vaccine can effectively enhance the levels of IL- 8 and IL- 12 in serum.
出处
《中国兽药杂志》
北大核心
2016年第5期11-14,共4页
Chinese Journal of Veterinary Drug
