中国4省市7～12岁儿童肥胖与端粒长度的关联

The relationship between obesity and telomere length in 7- 12 years old children in four provinces

目的探讨7-12岁儿童肥胖与端粒长度的关系,为正确评价儿童肥胖的潜在危害和完善儿童肥胖干预方案提供理论依据。方法在2012年卫生行业科研专项基线数据库中按体质量指数（BMI）筛选湖南、天津、辽宁、上海4省市7-12岁儿童253名,选取其身高、体重、腰围、臀围和血压等指标,采用实时荧光定量PCR方法测定其外周血白细胞T/S值,采用多元线性回归,分析身体测量指标与端粒长度的关系。结果女生平均T/S值（1.11±0.19）高于男生（1.06±0.18）,差异有统计学意义（t=-2.015,P〈0.05）,端粒长度在不同年龄组[〈10岁组（1.09±0.20）,≥10岁组（1.07±0.16）]和不同BMI组[体重正常组（1.09±0.20）,超重肥胖组（1.08±0.18）]间差异均无统计学意义（t值分别为0.811,0.468,P值均〉0.05）。逐步回归分析发现在男生中,端粒长度与臀围呈正相关（B=0.003,SE=0.001,P=0.034）,而女生中未发现端粒长度与身体测量指标相关。结论我国4省市儿童端粒长度存在性别差异,未发现端粒长度与肥胖和年龄的关联。

Objective To analysis the relationship between childhood obesity and telomere length by measuring the average leukocyte telomere length（ LTL）. Methods LTL was measured in 253 China children aged 7- 12 year（ 81 normal weight and 172 overweight / obese） using multiplex quantitative real-time PCR. The average LTL in the two groups was compared. The roles of anthropometrics index in predicting LTL were analyzed by multiple linear regressions. Results Boys had significantly shorter leukocyte telomeres than girls（ T / S ratio： 1. 06 ± 0. 18 vs. 1. 11 ± 0. 19）. No association between LTL and different BMI groups was found, neither do LTL and age groups（ 〈10 yr and ≥10 yr）. In boys, hip circumference（ β = 0. 003,SE = 0. 001,P = 0. 034）was associated with telomere length, but no significance was observed between LTL and other indices. There was no significant association between anthropometric indices with LTL in girls. Conclusion Boys have significantly shorter leukocyte telomeres than girls, but no evidence support the association between obesity and LTL among Chinese children aged 7- 12 years.