ZNRF3在甲状腺肿瘤组织中的表达以及在甲状腺癌细胞中的功能研究

The expression of ZNRF3 in thyroid cancer and its functions in thyroid cells

目的检测ZNRF3基因在不同分化程度甲状腺癌组织标本和细胞中的表达情况并探讨其在甲状腺癌中的作用。方法应用免疫组织化学技术检测35例乳头状甲状腺癌和10例低分化甲状腺癌组织中ZNRF3蛋白的表达。lit—PCR检测ZNRF3基因在乳头状甲状腺癌TPC-1和低分化甲状腺癌8505C细胞株中的表达。通过慢病毒沉默TPC1细胞中的ZNRF3基因，CCK-8与Transwell分别检测ZNRF3基因沉默后TPC-1细胞株增殖和侵袭迁移情况。结果免疫组织化学和RT-PCR分别检测发现，ZNRF3基因在乳头状甲状腺癌组织及细胞株中的表达明显高于低分化甲状腺癌组织及细胞，差异有统计学意义（4．83±0．44：3．13±0．59，t=2．20，P〈0．05；1．01±0．06：0．21±0．04，t=11．80，P〈0．01）。将TPC·1细胞株中ZNRF3基因沉默后，通过CCK-8检测细胞增殖发现，细胞培养72h后细胞增殖能力显著增强，差异有统计学意义（0．96±0．10：0．64±0．05，t=3．19，P〈0．05）；并且Transwell检测细胞侵袭与转移发现，细胞侵袭（0．12±0．01：0．09±0．00，t=5．48，P〈0．01）与迁移能力（0．22±0．01：0．17±0．01，t=4．58，P〈0．05）有所增强，差异有统计学意义。结论ZNRF3在乳头状甲状腺癌中的表达高于低分化甲状腺癌，ZNRF3在甲状腺肿瘤的发生发展中为抑癌基因。

Objective To detect the expression and function of ZNRF3 in different kinds of thyroid cancer tissues and cells. Methods Immunohistochemistry was used to detect the expressions of ZNRF3 protein in 35 cases of papillary thyroid carcinoma and 10 cases of poorly differentiated thyroid carcinoma. The expre- ssions of ZNRF3 gene in TPC-1 and 8505C were detected by RT-PCR, and the cell lines were derived from papillary thyroid carcinoma and poorly differentiated thyroid carcinoma respectively. After silenced ZNRF3 gene expression with lentiyirus, the proliferation ability of TPC-1 cells were detected with CCK-8, the invasion and metastasis ability of TPC-1 cells were detected with Transwell. Results According to results of immunohistochemistry and RT-PCR, the expressions of ZNRF3 in papillary thyroid carcinoma cells and tissues were higher than those in poorly differentiated thyroid carcinoma cells and tissues, the differences were statistically significant （4.83±0.44vs. 3.13±0.59, t =2.20, P 〈0.05; 1.01 ±0.06 vs. 0.21 ±0.04, t =11.80, P〈0. 01 ）. After ZNRF3 geng silencing, according to the results of CCK8, the proliferation ability of TPC-1 cells was significantly enhanced in 72 h, the difference was statistically significant （0.96 ± 0. 10 vs.0. 64 ± 0. 05, t =3.19, P〈0.05） ; and according to the results of Transwell, the TPC-1 cell＇s invasion （0.12±0.01 vs. 0. 09 ± 0.00, t = 5.48, P 〈 0.01 ） and migration （0.22 ± 0.01 vs. 0. 17 ±0.01, t = 4.58, P 〈 0.05 ） also increased, the differenees were statistically significant. Conclusion The expression of ZNRF3 in papillary thy- roid carcinoma is higher than that in poorly differentiated thyroid cancer. ZNRF＇3 is tumor suppressor gene in the thyroid tumors.