卵巢癌患者外周血清微小RNA-7表达及其临床意义

Expression and clinical significance of microRNA-7 in serum of ovarian cancer patients

目的 探讨微小RNA-7（miR-7）在卵巢癌患者血清中的表达及其临床意义。方法收集广东省佛山市南海区第六人民医院妇科2011年1月至2012年1月经病理组织学确诊的42例卵巢癌患者和40例在该院进行体检的健康女性的血清，采用反转录聚合酶链反应（RT-PCR）分别检测两组血清样本miR-7的表达，并分析其表达与卵巢癌临床特征的关系。结果与健康对照组相比，卵巢癌患者血清中miR-7表达显著下降（0．246±0．017：0．488±0．042），差异有统计学意义（t=11．23，P=0．01）。miR-7的表达与患者临床分期（t=10．12，P=0．01）、组织学类型（t=6．90，P=0．02）、分化程度（t=4．46，P=0．03）、区域淋巴结或远处转移（t=5．62，P=0．02）有关，而与患者的年龄（t=0．03，P=0．83）无关。生存期分析结果显示，miR-7高表达的卵巢癌患者总生存期长于低表达组（36．7个月：24．3个月），差异有统计学意义（X2=6．04，P=0．02）。结论miR-7在卵巢癌患者血清中表达下调，有助于卵巢癌病情评估，可能是卵巢癌患者重要的预后指标之一。

Objective To explore the expression and clinical significance of microRNA-7 （miR-7） in serum of ovarian cancer patients. Methods Serum samples of 42 ovarian patients confirmed by pathological histology and 40 healthy women who underwent a physical exam were collected from January 2011 to January 2012 in the Sixth People＇s Hospital of Foshan Nanhai District of Guangdong Province. Expression levels of miR-7 in the serum samples of the two groups were examined using reverse transcription-real time polymerase chain reaction （RT-PCR）. The relationship between the expression of miR-7 and the clinicopathologic feature of ovarian was analyzed. Results Compared with the controls, the expression of miR-7 in the serum of ovarian cancer patients was significantly reduced （ 0. 246 ± 0. 017 vs. 0. 488 ± 0. 042 ）, with a significant difference （t = 11.23, P= 0.01 ）. The expression of miR-7 in the serum of ovarian cancer patients was related to the clinical stage （ t = 10.12, P = 0.01 ）, pathological type （ t = 6.90, P = 0.02 ） , differentiation degree （ t = 4.46, P = 0.03 ） , regional lymph node or distant metastasis （ t = 5.62, P = 0.02 ） , but it was not related to the age （ t = 0.03, P = 0.83 ）. The patients with high miR-7 expression had better overall survival than the patients with low miR-7 expression （36.7 months vs. 24.3 months）, with a significant difference （X2 =6.04, P = 0.02 ）. Conclusion The expression of miR-7 in serum of ovarian cancer patients is down regulated, which may be helpful for the overall assessment of ovarian carcinoma, miR-7 may be one of the important prog- nostic indicators for ovarian carcinoma.