生发酊对C57BL/6小鼠毛囊细胞凋亡的影响

Impact of hair-growth tincture on hair follicle cell apoptosis in animal model for C57BL/6 mice

目的从毛囊细胞凋亡的角度出发,观察生发酊对动物模型小鼠毛发生长周期的影响,评价其治疗脱发的效果。方法将C57BL/6小鼠随机分为生发酊组、阳性对照组、阴性对照组、模型对照组,制备小鼠脱发动物模型。脱毛后第2天,于脱毛区涂相应药物;脱毛后第17天,颈椎脱臼处死小鼠,背部平行脊柱相同部位取材,用原位杂交技术原位末端标记法(TUNEL)检测毛囊内细胞的凋亡状况。结果生发酊组[(15.82±0.36)个/视野]与阳性对照组[(16.12±0.39)个/视野]细胞凋亡数量明显少于阴性对照组[(40.32±0.47)个/视野]与模型对照组[(42.16±0.54)个/视野],差异有统计学意义(P<0.01);生发酊组与阳性对照组比较差异无统计学意义(P>0.05)。结论生发酊对毛囊细胞凋亡有明显的抑制作用,此为其治疗脱发的可能机制之一。

Objective To observe the impact of hair-growth tincture on mice hair growth cycle in animal model from the angle of hair follicle cell apoptosis and to evaluate its effects in treatment hair loss. Methods C57BL/6 mice were randomly divided into hair-growth tincture group,positive control group,negative control group and model control group and then mice hair loss model were made.Corresponding drugs were smeared to hair loss area from the second day after hair loss.Mice were killed by cervical dislocation at the 17 day after hair loss.The samples were draw from same part of back parallel spine.In situ hybridization TUNEL was used to test the apoptosis condition of hair follicle cell. Results The apoptosis number in hair-growth tincture group and positive control group with a view of（15.82 ±0.36） cells and（16.12 ±0.39） cells were obvious less than those in negative control group and model control group with a view of（40.32±0.47） cells and（42.16±0.54） cells,and the differences were statistical significant（P0.01）.The difference between hair-growth tincture group and positive control group had no statistical significance（P0.05）. Conclusion Hairgrowth tincture has obvious role in inhibiting hair follicle cell apoptosis and this may be one of the mechanism in treating hair loss.