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孟鲁司特中间体酶促转化反应的HPLC测定方法

HPLC Method for Analyzing Enzamatic Transformation of Montelukast Intermediate
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摘要 建立了孟鲁司特中间体酶促转化反应中底物和产物浓度变化的HPLC测定方法。采用C18反相色谱柱，以乙腈和0．1％磷酸（80：20）为流动相，检测波长为225nm。结果表明，底物和产物浓度在50～400μg／ml范围内线性关系良好，适用于孟鲁司特中间体酶促反应的监控。 HPLC method was established to analyze enzamatic transformation of montelukast intermediate. The column was Cosmosil 5C18-MS-II, the mobile phase was acetonitrile and 0.1% phosphoric acid （80：20）, and the detection wavlength was 225 nm. The results indicates that both the substrate and product of the enzymatic transformation show good linear relation at the range of 50-400 μg/ml, and the method is suitable for the control of enzymatic transformation of montelukast intermediate.
作者 沈露梦 钟永军 施珊珊 周超阳 杨仲毅
机构地区 台州学院生命科学学院
出处 《台州学院学报》 2016年第3期15-18,22,共5页 Journal of Taizhou University
关键词 孟鲁司特中间体 酮还原酶 生物催化 高效液相色谱 Montelukast intermediate Ketone reductase Biocatalyst HPL
分类号 R927.2 [医药卫生—药学] O657.72 [理学—分析化学]
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台州学院学报
2016年 第3期

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