Transwell小室环境下小鼠不同器官微环境对人舌癌细胞生长的影响

The effect of different organs microenvironments of mice on the growth of human tongue cancer cells in a Transwell cell insert

目的:探讨骨髓和淋巴结微环境对人舌癌细胞SCC-9生长的影响。方法:利用Transwell小室建立微环境与SCC-9共培养体外模型,分为5组:SCC-9单独培养组(对照组),SCC-9+Balb/c小鼠骨髓共培养组,SCC-9+Balb/c小鼠淋巴结共培养组,SCC-9+Balb/c裸鼠骨髓共培养组,SCC-9+Balb/c裸鼠淋巴结共培养组。采用直接计数法分别于24 h、48 h、72 h、96 h、120 h、144 h检测SCC-9的数量。结果:利用Transwell小室成功构建骨髓、淋巴结微环境与SCC-9共培养体外模型。计数结果显示,SCC-9在Balb/c裸鼠淋巴结共培养组中的增殖程度高于其他4组(P<0.05);在Balb/c裸鼠骨髓,Balb/c小鼠骨髓和淋巴结共培养组中均低于对照组(P<0.05),且抑制程度无明显差异(P>0.05)。结论:不同微环境可能通过不同的肿瘤休眠机制,控制肿瘤细胞的增殖与休眠,最终被动形成淋巴转移的靶向性。

Objective：To investigate the effect of bone marrow and lymph node microenvironment on the growth of human tongue squamous carcinoma cell line SCC-9. Method：Transwell cell insert was uesd to establish the microenvironment and SCC-9 co-cultured model in vitro. The experiment was divided into 5 groups： SCC-9 cultured alone group（control group）, SCC-9 and Balb/c mice bone marrow co-cultured group, SCC-9 and Balb/c mice lymph nodes co-cultured group,SCC-9 and Balb/c nude mice bone marrow co-cultured group, SCC-9 and Balb/c nude mice lymph nodes co-cultured group. The number of SCC-9 was counted by direct counting method at 24 h, 48 h, 72 h, 96 h, 120 h, 144 h. Result：The different microenvironments and SCC-9 co-cultured model in vitro was established by Transwell cell insert successfully. The proliferation of SCC-9 in Balb/c nude mice lymph node microenvironment was higher than those of the other four groups（P〈0.05）. There is no statistic difference among Balb/c mice bone marrow, nude mice bone marrow and nude mice lymph nodes microenvironment（P〈0.05）,in which the SCC-9 proliferations were lower than the control group（P〈0.05）. Conclusion：Different microenvironments may control the proliferation and dormancy of tumor cell by different tumor dormancy mechanisms,and cause the passive targeted lymphatic metastasis.